LOCUS dna 8274 bp FEATURES Location/Qualifiers Promoter 176..400 /gene="URA3 prom" misc_binding 314..319 /dbxref="REBASE:NdeI" Marker 402..1202 /gene="URA3 marker" misc_binding 588..593 /dbxref="REBASE:EcoRV" misc_binding 776..781 /dbxref="REBASE:ApaI" misc_binding 836..841 /dbxref="REBASE:StuI" Rep_Origin 1468..1774 /gene="f1 origin" Reporter 1796..1954 /gene="lacZ-alhpa reporter" Promoter 1962..1981 /gene="T7 prom" misc_binding 1989..1994 /dbxref="REBASE:KpnI" Tag 2023..2370 /gene="protA_analogue tag" misc_binding 2470..2475 /dbxref="REBASE:XbaI" Other Gene 2488..2607 /gene="attR1 other" Other Gene 2507..2607 /gene="attR2 other" Other Gene 2648..2953 /gene="ccdB other" misc_binding 2867..2872 /dbxref="REBASE:AvrI" misc_binding 2867..2872 /dbxref="REBASE:SmaI" misc_binding 2867..2872 /dbxref="REBASE:AvaI" misc_binding 2867..2872 /dbxref="REBASE:XmaI" Marker 3295..3954 /gene="ChlorR marker" misc_binding 3736..3741 /dbxref="REBASE:EcoRI" misc_binding 4055..4062 /dbxref="REBASE:NotI" Other Gene 4063..4187 /gene="attR1 other" Other Gene 4063..4163 /gene="attR2 other" misc_binding 4653..4658 /dbxref="REBASE:SacI" Promoter 4670..4689 /gene="T3 prom" Promoter 4753..4836 /gene="lac prom" Rep_Origin 5097..5716 /gene="pUC origin" Marker 5871..6731 /gene="AmpR marker" Promoter 6773..6811 /gene="Bacterial-Promoter prom" misc_binding 6913..6918 /dbxref="REBASE:HpaI" BASE COUNT 2264 a 1787 c 1892 g 2331 t 0 others ORIGIN 1 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 61 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 121 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat accacagctt 181 ttcaattcaa ttcatcattt tttttttatt cttttttttg atttcggttt ctttgaaatt 241 tttttgattc ggtaatctcc gaacagaagg aagaacgaag gaaggagcac agacttagat 301 tggtatatat acgcatatgt agtgttgaag aaacatgaaa ttgcccagta ttcttaaccc 361 aactgcacag aacaaaaacc tgcaggaaac gaagataaat catgtcgaaa gctacatata 421 aggaacgtgc tgctactcat cctagtcctg ttgctgccaa gctatttaat atcatgcacg 481 aaaagcaaac aaacttgtgt gcttcattgg atgttcgtac caccaaggaa ttactggagt 541 tagttgaagc attaggtccc aaaatttgtt tactaaaaac acatgtggat atcttgactg 601 atttttccat ggagggcaca gttaagccgc taaaggcatt atccgccaag tacaattttt 661 tactcttcga agacagaaaa tttgctgaca ttggtaatac agtcaaattg cagtactctg 721 cgggtgtata cagaatagca gaatgggcag acattacgaa tgcacacggt gtggtgggcc 781 caggtattgt tagcggtttg aagcaggcgg cagaagaagt aacaaaggaa cctagaggcc 841 ttttgatgtt agcagaattg tcatgcaagg gctccctatc tactggagaa tatactaagg 901 gtactgttga cattgcgaag agcgacaaag attttgttat cggctttatt gctcaaagag 961 acatgggtgg aagagatgaa ggttacgatt ggttgattat gacacccggt gtgggtttag 1021 atgacaaggg agacgcattg ggtcaacagt atagaaccgt ggatgatgtg gtctctacag 1081 gatctgacat tattattgtt ggaagaggac tatttgcaaa gggaagggat gctaaggtag 1141 agggtgaacg ttacagaaaa gcaggctggg aagcatattt gagaagatgc ggccagcaaa 1201 actaaaaaac tgtattataa gtaaatgcat gtatactaaa ctcacaaatt agagcttcaa 1261 tttaattata tcagttatta ccctatgcgg tgtgaaatac cgcacagatg cgtaaggaga 1321 aaataccgca tcaggaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt 1381 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa 1441 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa 1501 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac 1561 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga 1621 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa 1681 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc 1741 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcgcgccatt 1801 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac 1861 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt 1921 cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg 1981 gcgaattggg taccccggcg cgcctcactg atgattcgcg tctactttcg gcgcctgagc 2041 atcatttagc tttttagctt ctgctaaaag gttagcgctt tggcttgggt catcttttaa 2101 actttggatg aaggcgtttc gttgttcttc gtttaagtta ggtaaatgta agatctcata 2161 gaacgcgttt tgttgttctt tgttgaattt gttgtctact ttcggcgcct gagcatcatt 2221 tagcttttta gcttctgcta aaaggttagc gctttggctt gggtcatctt ttaaactttg 2281 gatgaaggcg tttcgttgtt cttcgtttaa gttaggtaaa tgtaagatct catagaacgc 2341 gttttgttgt tctttgttga atttgttgtc cacggccgat ggtccaggac cttgaaaaag 2401 aacttctaaa ccagcataat caggaacatc gtatggataa aagattctac catgatggtg 2461 atgatgatgt ctagacacat caaccacttt gtacaagaaa gctgaacgag aaacgtaaaa 2521 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg 2581 taaaacacaa catatccagt cactatggtc gacctgcaga ctggctgtgt ataagggagc 2641 ctgacattta tattccccag aacatcaggt taatggcgtt tttgatgtca ttttcgcggt 2701 ggctgagatc agccacttct tccccgataa cggagaccgg cacactggcc atatcggtgg 2761 tcatcatgcg ccagctttca tccccgatat gcaccaccgg gtaaagttca cgggagactt 2821 tatctgacag cagacgtgca ctggccaggg ggatcaccat ccgtcgcccg ggcgtgtcaa 2881 taatatcact ctgtacatcc acaaacagac gataacggct ctctctttta taggtgtaaa 2941 ccttaaactg catttcacca gtccctgttc tcgtcagcaa aagagccgtt catttcaata 3001 aaccgggcga cctcagccat cccttcctga ttttccgctt tccagcgttc ggcacgcaga 3061 cgacgggctt cattctgcat ggttgtgctt accagaccgg agatattgac atcatatatg 3121 ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt aatacgctgc ttcatagcac 3181 acctcttttt gacatacttc gggtatacat atcagtatat attcttatac cgcaaaaatc 3241 agcgcgcaaa tacgcatact gttatctggc ttttagtaag ccggatccag atctttacgc 3301 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa 3361 gccatcacag acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg 3421 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt 3481 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat 3541 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat 3601 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt 3661 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc 3721 tttcattgcc atacggaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa 3781 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag 3841 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt 3901 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc 3961 ttccttagct cctgaaaatc tcgccggatc ctaactcaaa atccacacat tatacgagcc 4021 ggaagcataa agtgtaaagc ctggggtgcc taatgcggcc gccatagtga ctggatatgt 4081 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata 4141 tttatatcat tttacgtttc tcgttcagct tttttgtaca aacttgttga taattccttc 4201 tccttgacgt taaagtatag aggtatatta acaatttttt gttgatactt ttattacatt 4261 tgaataagaa gtaatacaaa ccgaaaatgt tgaaagtatt agttaaagtg gttatgcagt 4321 ttttgcattt atatatctgt taatagatca aaaatcatcg cttcgctgat taattacccc 4381 agaaataagg ctaaaaaact aatcgcatta tcatcctatg gttgttaatt tgattcgttc 4441 atttgaaggt ttgtggggcc aggttactgc caatttttcc tcttcataac cataaaagct 4501 agtattgtag aatctttatt gttcggagca gtgcggcgcg aggcacatct gcgtttcagg 4561 aacgcgaccg gtgaagacga ggacgcacgg aggagagtct tccttcggag ggctgtcacc 4621 cgctcggcgg cttctaatcc gttcccgcgg tggagctcca gcttttgttc cctttagtga 4681 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 4741 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 4801 taatgagtga ggtaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 4861 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 4921 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 4981 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 5041 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 5101 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 5161 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 5221 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 5281 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 5341 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 5401 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 5461 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 5521 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 5581 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 5641 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 5701 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 5761 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 5821 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 5881 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 5941 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 6001 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 6061 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 6121 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 6181 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 6241 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 6301 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 6361 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 6421 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 6481 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 6541 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 6601 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 6661 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 6721 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 6781 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 6841 tttccccgaa aagtgccacc tgtatactag gttatagttt cctttactat cgtaacttcc 6901 tactctgatt aggttaactc ctcaccgtcg tatatcttgt cgatttccca tcacgacttc 6961 cttcgtatgc tatggggcgt accttaccct attatagtgt cctccatgat ctgatggaaa 7021 gtaggatgta tttatctgcg tatattcatg cgtaaattcg tatttgtgcg tgatacggca 7081 agaagagtac atatatatat atgtccgttg tgcgtctata tccacgctgc acttgtcact 7141 cgacatacac gcgtcgagcg caacgtaaaa gccttcgcga gcaaaagcct ttgcgaaact 7201 tcaaggataa ggcttcaagg ataagagatc tttcatatcc ttgaagtctc gcgaaaactt 7261 ttggttttcg cgagacttct gcgtgaaagt tttttggttt ttgcgtggcc tgacattgct 7321 cgatgatttt ataacgctta tggcgaaggt gtttgtaacg agttttcata gagaaacgat 7381 atatagagac acgatatagg gatatattgg atgggtaggt ggaaagcgag gaacttgaac 7441 gtagatttga gctggagatg taaaaaatac aaatagagat cataatgaga aatctgtttt 7501 tttaacatca ttcttgataa gtatctcact tagcttttgt tatgctttta catttgtaaa 7561 ggatatgcat catatatctc tgttttatct tctttggcaa gtattaaaag actggttact 7621 tcttagtagt tgcgatagtg aaagacaagt gtttcatacg cgttaggtgt agccatatct 7681 tatattagcc cctacggaaa tagaactttt ttacgtgggc gtcgaagcga tcattagtca 7741 tttgcgccct tcacctcagt ccgaaaaaaa taccttctct tttatctgtg gtttcatcgg 7801 aagaagattg gaattgcctg gatgtcacgt ttttcaatag ttctctgacg taatatctcg 7861 cgtgtttcct ctttttttca ttagattcta cgaaacaatc tttttatcgc gagagcccta 7921 cgtaaaaaca tcttgttttt tcttcatatc taagaaacaa ccattttatc gcgagagcgc 7981 aacgtaaaga caagacattt ttacgtcgag tctaagaaac aaacttttta atcgcgagag 8041 cgcaacgtaa aaacaaaatg tttttacttc gtgtctaaga agcaaccatt ttatcgcgaa 8101 agcgcaacgt aaagacaaga catttttacg tcgagtctaa gaaacaaact ttttaatcgc 8161 gagagcgcaa cgtaaaaaca agatgtttta cttcgtgtct acgaagcaac taagaaacca 8221 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc //