LOCUS dna 8274 bp FEATURES Location/Qualifiers Promoter 11..455 /gene="GAL1 prom" Other Gene 466..590 /gene="attR1 other" misc_binding 591..598 /dbxref="REBASE:NotI" Marker 699..1358 /gene="ChlorR marker" misc_binding 912..917 /dbxref="REBASE:EcoRI" Marker 1700..2005 /gene="ccdB marker" misc_binding 1781..1786 /dbxref="REBASE:AvrI" misc_binding 1781..1786 /dbxref="REBASE:SmaI" misc_binding 1781..1786 /dbxref="REBASE:AvaI" misc_binding 1781..1786 /dbxref="REBASE:XmaI" Other Gene 2046..2170 /gene="attR2 other" misc_binding 2178..2183 /dbxref="REBASE:XbaI" Tag 2184..2202 /gene="6xHis tag" Tag 2214..2240 /gene="HA tag" Other Gene 2244..2267 /gene="3C-recognition other" Tag 2283..2630 /gene="protA_analogue tag" misc_binding 2659..2664 /dbxref="REBASE:KpnI" Promoter 2672..2691 /gene="T7 prom" Reporter 2699..2857 /gene="lacZ-alhpa reporter" Rep_Origin 2879..3185 /gene="f1 origin" Marker 3451..4251 /gene="URA3 marker" misc_binding 3812..3817 /dbxref="REBASE:StuI" misc_binding 3872..3877 /dbxref="REBASE:ApaI" misc_binding 4060..4065 /dbxref="REBASE:EcoRV" Promoter 4253..4477 /gene="URA3 prom" misc_binding 4334..4339 /dbxref="REBASE:NdeI" misc_binding 6009..6014 /dbxref="REBASE:HpaI" Promoter 6116..6154 /gene="Bact prom" Marker 6196..7056 /gene="AmpR marker" Rep_Origin 7211..7830 /gene="pUC origin" misc_binding 8269..8274 /dbxref="REBASE:SacI" BASE COUNT 2331 a 1892 c 1787 g 2264 t 0 others ORIGIN 1 caccgcggga acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc 61 ctccgtgcgt cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc 121 actgctccga acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat 181 tggcagtaac ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat 241 gataatgcga ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt 301 tttgatctat taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt 361 caacattttc ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt 421 gttaatatac ctctatactt taacgtcaag gagaaggaat tatcaacaag tttgtacaaa 481 aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat 541 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg gcggccgcat 601 taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg attttgagtt 661 aggatccggc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat 721 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag 781 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg 841 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga 901 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg 961 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg 1021 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg 1081 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca 1141 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg 1201 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg 1261 cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg cttaatgaat 1321 tacaacagta ctgcgatgag tggcagggcg gggcgtaaag atctggatcc ggcttactaa 1381 aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga atatatactg 1441 atatgtatac ccgaagtatg tcaaaaagag gtgtgctatg aagcagcgta ttacagtgac 1501 agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg 1561 gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa 1621 aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac 1681 gagaacaggg actggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta 1741 tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat 1801 ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt 1861 gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc 1921 cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat 1981 taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg 2041 tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca 2101 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt 2161 acaaagtggt tgatgtgtct agacatcatc atcaccatca tggtagaatc ttttatccat 2221 acgatgttcc tgattatgct ggtttagaag ttctttttca aggtcctgga ccatcggccg 2281 tggacaacaa attcaacaaa gaacaacaaa acgcgttcta tgagatctta catttaccta 2341 acttaaacga agaacaacga aacgccttca tccaaagttt aaaagatgac ccaagccaaa 2401 gcgctaacct tttagcagaa gctaaaaagc taaatgatgc tcaggcgccg aaagtagaca 2461 acaaattcaa caaagaacaa caaaacgcgt tctatgagat cttacattta cctaacttaa 2521 acgaagaaca acgaaacgcc ttcatccaaa gtttaaaaga tgacccaagc caaagcgcta 2581 accttttagc agaagctaaa aagctaaatg atgctcaggc gccgaaagta gacgcgaatc 2641 atcagtgagg cgcgccgggg tacccaattc gccctatagt gagtcgtatt acgcgcgctc 2701 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg 2761 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg 2821 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc gacgcgccct gtagcggcgc 2881 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 2941 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 3001 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 3061 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 3121 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 3181 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 3241 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 3301 attaacgttt acaatttcct gatgcggtat tttctcctta cgcatctgtg cggtatttca 3361 caccgcatag ggtaataact gatataatta aattgaagct ctaatttgtg agtttagtat 3421 acatgcattt acttataata cagtttttta gttttgctgg ccgcatcttc tcaaatatgc 3481 ttcccagcct gcttttctgt aacgttcacc ctctacctta gcatcccttc cctttgcaaa 3541 tagtcctctt ccaacaataa taatgtcaga tcctgtagag accacatcat ccacggttct 3601 atactgttga cccaatgcgt ctcccttgtc atctaaaccc acaccgggtg tcataatcaa 3661 ccaatcgtaa ccttcatctc ttccacccat gtctctttga gcaataaagc cgataacaaa 3721 atctttgtcg ctcttcgcaa tgtcaacagt acccttagta tattctccag tagataggga 3781 gcccttgcat gacaattctg ctaacatcaa aaggcctcta ggttcctttg ttacttcttc 3841 tgccgcctgc ttcaaaccgc taacaatacc tgggcccacc acaccgtgtg cattcgtaat 3901 gtctgcccat tctgctattc tgtatacacc cgcagagtac tgcaatttga ctgtattacc 3961 aatgtcagca aattttctgt cttcgaagag taaaaaattg tacttggcgg ataatgcctt 4021 tagcggctta actgtgccct ccatggaaaa atcagtcaag atatccacat gtgtttttag 4081 taaacaaatt ttgggaccta atgcttcaac taactccagt aattccttgg tggtacgaac 4141 atccaatgaa gcacacaagt ttgtttgctt ttcgtgcatg atattaaata gcttggcagc 4201 aacaggacta ggatgagtag cagcacgttc cttatatgta gctttcgaca tgatttatct 4261 tcgtttcctg caggtttttg ttctgtgcag ttgggttaag aatactgggc aatttcatgt 4321 ttcttcaaca ctacatatgc gtatatatac caatctaagt ctgtgctcct tccttcgttc 4381 ttccttctgt tcggagatta ccgaatcaaa aaaatttcaa agaaaccgaa atcaaaaaaa 4441 agaataaaaa aaaaatgatg aattgaattg aaaagctgtg gtatggtgca ctctcagtac 4501 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc 4561 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 4621 gagctgcatg tgtcagaggt tttcaccgtc atgacgaaag ggcctcgtga tacgcctatt 4681 tttataggtt aatgtcatga taataatggt ttcttagttg cttcgtagac acgaagtaaa 4741 acatcttgtt tttacgttgc gctctcgcga ttaaaaagtt tgtttcttag actcgacgta 4801 aaaatgtctt gtctttacgt tgcgctttcg cgataaaatg gttgcttctt agacacgaag 4861 taaaaacatt ttgtttttac gttgcgctct cgcgattaaa aagtttgttt cttagactcg 4921 acgtaaaaat gtcttgtctt tacgttgcgc tctcgcgata aaatggttgt ttcttagata 4981 tgaagaaaaa acaagatgtt tttacgtagg gctctcgcga taaaaagatt gtttcgtaga 5041 atctaatgaa aaaaagagga aacacgcgag atattacgtc agagaactat tgaaaaacgt 5101 gacatccagg caattccaat cttcttccga tgaaaccaca gataaaagag aaggtatttt 5161 tttcggactg aggtgaaggg cgcaaatgac taatgatcgc ttcgacgccc acgtaaaaaa 5221 gttctatttc cgtaggggct aatataagat atggctacac ctaacgcgta tgaaacactt 5281 gtctttcact atcgcaacta ctaagaagta accagtcttt taatacttgc caaagaagat 5341 aaaacagaga tatatgatgc atatccttta caaatgtaaa agcataacaa aagctaagtg 5401 agatacttat caagaatgat gttaaaaaaa cagatttctc attatgatct ctatttgtat 5461 tttttacatc tccagctcaa atctacgttc aagttcctcg ctttccacct acccatccaa 5521 tatatcccta tatcgtgtct ctatatatcg tttctctatg aaaactcgtt acaaacacct 5581 tcgccataag cgttataaaa tcatcgagca atgtcaggcc acgcaaaaac caaaaaactt 5641 tcacgcagaa gtctcgcgaa aaccaaaagt tttcgcgaga cttcaaggat atgaaagatc 5701 tcttatcctt gaagccttat ccttgaagtt tcgcaaaggc ttttgctcgc gaaggctttt 5761 acgttgcgct cgacgcgtgt atgtcgagtg acaagtgcag cgtggatata gacgcacaac 5821 ggacatatat atatatgtac tcttcttgcc gtatcacgca caaatacgaa tttacgcatg 5881 aatatacgca gataaataca tcctactttc catcagatca tggaggacac tataataggg 5941 taaggtacgc cccatagcat acgaaggaag tcgtgatggg aaatcgacaa gatatacgac 6001 ggtgaggagt taacctaatc agagtaggaa gttacgatag taaaggaaac tataacctag 6061 tatacaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 6121 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 6181 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 6241 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 6301 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 6361 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 6421 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 6481 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 6541 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 6601 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 6661 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 6721 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 6781 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 6841 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 6901 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 6961 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 7021 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 7081 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 7141 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 7201 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 7261 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 7321 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta 7381 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 7441 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 7501 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 7561 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 7621 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 7681 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 7741 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 7801 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 7861 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 7921 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 7981 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 8041 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 8101 attaatgtga gttacctcac tcattaggca ccccaggctt tacactttat gcttccggct 8161 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat 8221 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctc //