LOCUS       dna                      3519 bp                                   
FEATURES             Location/Qualifiers
     Promoter        95..178
                     /gene="lac prom"
     Reporter        217..576
                     /gene="lacZ-alpha2 reporter"
     misc_binding    276..281
                     /dbxref="REBASE:HindIII"
     misc_binding    282..287
                     /dbxref="REBASE:KpnI"
     misc_binding    288..293
                     /dbxref="REBASE:SacI"
     misc_binding    294..299
                     /dbxref="REBASE:BamHI"
     misc_binding    348..353
                     /dbxref="REBASE:PstI"
     misc_binding    353..358
                     /dbxref="REBASE:EcoRV"
     misc_binding    369..376
                     /dbxref="REBASE:NotI"
     misc_binding    376..381
                     /dbxref="REBASE:XhoI"
     misc_binding    388..393
                     /dbxref="REBASE:XbaI"
     misc_binding    394..399
                     /dbxref="REBASE:ApaI"
     Promoter        406..425
                     /gene="T7 prom"
     Other Gene      585..888
                     /gene="ccdB other"
     misc_binding    1206..1211
                     /dbxref="REBASE:BclI"
     Marker          1240..2028
                     /gene="NeoR marker"
     Promoter        2155..2193
                     /gene="Bacterial-Promoter prom"
     misc_binding    2231..2243
                     /dbxref="REBASE:SfiI"
     Marker          2238..2612
                     /gene="ZeoR marker"
     misc_binding    2246..2251
                     /dbxref="REBASE:HincII"
     misc_binding    2326..2331
                     /dbxref="REBASE:SmaI"
     misc_binding    2326..2331
                     /dbxref="REBASE:XmaI"
     misc_binding    2637..2642
                     /dbxref="REBASE:DraI"
     Rep_Origin      2734..3353
                     /gene="pUC origin"
BASE COUNT    780 a   935 c   1004 g   800 t    0 others
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc     
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc     
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa     
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat     
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca     
      301 ctagtaacgg ccgccagtgt gctggaattc gcccttaagg gcgaattctg cagatatcca     
      361 tcacactggc ggccgctcga gcatgcatct agagggccca attcgcccta tagtgagtcg     
      421 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc     
      481 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc     
      541 cgcaccgatc gcccttccca acagttgcgc agcctatacg tacggcagtt taaggtttac     
      601 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac     
      661 acgccggggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc     
      721 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc     
      781 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc     
      841 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggcatg     
      901 agattatcaa aaaggatctt cacctagatc cttttcacgt agaaagccag tccgcagaaa     
      961 cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc     
     1021 gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt     
     1081 ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag     
     1141 ccctgcaaag taaactggat ggctttctcg ccgccaagga tctgatggcg caggggatca     
     1201 agctctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac     
     1261 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca     
     1321 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt     
     1381 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg     
     1441 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga     
     1501 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct     
     1561 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg     
     1621 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg     
     1681 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc     
     1741 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat     
     1801 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac     
     1861 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt     
     1921 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct     
     1981 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg aattattaac     
     2041 gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc     
     2101 atacaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa     
     2161 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatagc     
     2221 acgtgaggag ggccaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg     
     2281 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg     
     2341 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg     
     2401 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt     
     2461 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga     
     2521 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact     
     2581 gcgtgcactt cgtggccgag gagcaggact gacacgtgct aaaacttcat ttttaattta     
     2641 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt     
     2701 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt     
     2761 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt     
     2821 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc     
     2881 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg     
     2941 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg     
     3001 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt     
     3061 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac     
     3121 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg     
     3181 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg     
     3241 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat     
     3301 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt     
     3361 tacggttcct gggcttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg     
     3421 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa     
     3481 cgaccgagcg cagcgagtca gtgagcgagg aagcggaag                            
//