1 ctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctga 60 GAAAGGACGCAATAGGGGACTAAGACACCTATTGGCATAATGGCGGAAACTCACTCGACT 61 taccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaaga 120 ATGGCGAGCGGCGTCGGCTTGCTGGCTCGCGTCGCTCAGTCACTCGCTCCTTCGCCTTCT 121 gcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggca 180 CGCGGGTTATGCGTTTGGCGGAGAGGGGCGCGCAACCGGCTAAGTAATTACGTCGACCGT 181 cgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatacgcgtaccgc 240 GCTGTCCAAAGGGCTGACCTTTCGCCCGTCACTCGCGTTGCGTTAATTATGCGCATGGCG T1(transcriptional-stop) reg(268,295)<<< | 241 tagccaggaagagtttgtagaaacgcaaaaaggccatccgtcaggatggccttctgctta 300 ATCGGTCCTTCTCAAACATCTTTGCGTTTTTCCGGTAGGCAGTCCTACCGGAAGACGAAT 301 gtttgatgcctggcagtttatggcgggcgtcctgcccgccaccctccgggccgttgcttc 360 CAAACTACGGACCGTCAAATACCGCCCGCAGGACGGGCGGTGGGAGGCCCGGCAACGAAG 361 acaacgttcaaatccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaa 420 TGTTGCAAGTTTAGGCGAGGGCCGCCTAAACAGGATGAGTCCTCTCGCAAGTGGCTGTTT T2(transcriptional-stop) reg(427,470)<<< | 421 caacagataaaacgaaaggcccagtcttccgactgagcctttcgttttatttgatgcctg 480 GTTGTCTATTTTGCTTTCCGGGTCAGAAGGCTGACTCGGAAAGCAAAATAAACTACGGAC HpaI | 481 gcagttccctactctcgcgttaacgctagcatggatgttttcccagtcacgacgttgtaa 540 CGTCAAGGGATGAGAGCGCAATTGCGATCGTACCTACAAAAGGGTCAGTGCTGCAACATT attP other(570,801)>>> | ApaI | | 541 aacgacggccagtcttaagctcgggccccaaataatgattttattttgactgatagtgac 600 TTGCTGCCGGTCAGAATTCGAGCCCGGGGTTTATTACTAAAATAAAACTGACTATCACTG 601 ctgttcgttgcaacaaattgatgagcaatgcttttttataatgccaactttgtacaaaaa 660 GACAAGCAACGTTGTTTAACTACTCGTTACGAAAAAATATTACGGTTGAAACATGTTTTT 661 agctgaacgagaaacgtaaaatgatataaatatcaatatattaaattagattttgcataa 720 TCGACTTGCTCTTTGCATTTTACTATATTTATAGTTATATAATTTAATCTAAAACGTATT 721 aaaacagactacataatactgtaaaacacaacatatccagtcactatgaatcaactactt 780 TTTTGTCTGATGTATTATGACATTTTGTGTTGTATAGGTCAGTGATACTTAGTTGATGAA 781 agatggtattagtgacctgtagtcgaccgacagccttccaaatgttcttcgggtgatgct 840 TCTACCATAATCACTGGACATCAGCTGGCTGTCGGAAGGTTTACAAGAAGCCCACTACGA 841 gccaacttagtcgaccgacagccttccaaatgttcttctcaaacggaatcgtcgtatcca 900 CGGTTGAATCAGCTGGCTGTCGGAAGGTTTACAAGAAGAGTTTGCCTTAGCAGCATAGGT 901 gcctactcgctattgtcctcaatgccgtattaaatcataaaaagaaataagaaaaagagg 960 CGGATGAGCGATAACAGGAGTTACGGCATAATTTAGTATTTTTCTTTATTCTTTTTCTCC 961 tgcgagcctcttttttgtgtgacaaaataaaaacatctacctattcatatacgctagtgt 1020 ACGCTCGGAGAAAAAACACACTGTTTTATTTTTGTAGATGGATAAGTATATGCGATCACA 1021 catagtcctgaaaatcatctgcatcaagaacaatttcacaactcttatacttttctctta 1080 GTATCAGGACTTTTAGTAGACGTAGTTCTTGTTAAAGTGTTGAGAATATGAAAAGAGAAT 1081 caagtcgttcggcttcatctggattttcagcctctatacttactaaacgtgataaagttt 1140 GTTCAGCAAGCCGAAGTAGACCTAAAAGTCGGAGATATGAATGATTTGCACTATTTCAAA PstI ccdB marker(1197,1502)<<< | | 1141 ctgtaatttctactgtatcgacctgcagactggctgtgtataagggagcctgacatttat 1200 GACATTAAAGATGACATAGCTGGACGTCTGACCGACACATATTCCCTCGGACTGTAAATA 1201 attccccagaacatcaggttaatggcgtttttgatgtcattttcgcggtggctgagatca 1260 TAAGGGGTCTTGTAGTCCAATTACCGCAAAAACTACAGTAAAAGCGCCACCGACTCTAGT 1261 gccacttcttccccgataacggagaccggcacactggccatatcggtggtcatcatgcgc 1320 CGGTGAAGAAGGGGCTATTGCCTCTGGCCGTGTGACCGGTATAGCCACCAGTAGTACGCG 1321 cagctttcatccccgatatgcaccaccgggtaaagttcacgggagactttatctgacagc 1380 GTCGAAAGTAGGGGCTATACGTGGTGGCCCATTTCAAGTGCCCTCTGAAATAGACTGTCG SmaI | XmaI | | 1381 agacgtgcactggccagggggatcaccatccgtcgcccgggcgtgtcaataatatcactc 1440 TCTGCACGTGACCGGTCCCCCTAGTGGTAGGCAGCGGGCCCGCACAGTTATTATAGTGAG 1441 tgtacatccacaaacagacgataacggctctctcttttataggtgtaaaccttaaactgc 1500 ACATGTAGGTGTTTGTCTGCTATTGCCGAGAGAGAAAATATCCACATTTGGAATTTGACG 1501 atttcaccagtccctgttctcgtcagcaaaagagccgttcatttcaataaaccgggcgac 1560 TAAAGTGGTCAGGGACAAGAGCAGTCGTTTTCTCGGCAAGTAAAGTTATTTGGCCCGCTG 1561 ctcagccatcccttcctgattttccgctttccagcgttcggcacgcagacgacgggcttc 1620 GAGTCGGTAGGGAAGGACTAAAAGGCGAAAGGTCGCAAGCCGTGCGTCTGCTGCCCGAAG 1621 attctgcatggttgtgcttaccagaccggagatattgacatcatatatgccttgagcaac 1680 TAAGACGTACCAACACGAATGGTCTGGCCTCTATAACTGTAGTATATACGGAACTCGTTG 1681 tgatagctgtcgctgtcaactgtcactgtaatacgctgcttcatagcacacctctttttg 1740 ACTATCGACAGCGACAGTTGACAGTGACATTATGCGACGAAGTATCGTGTGGAGAAAAAC 1741 acatacttcgggtatacatatcagtatatattcttataccgcaaaaatcagcgcgcaaat 1800 TGTATGAAGCCCATATGTATAGTCATATATAAGAATATGGCGTTTTTAGTCGCGCGTTTA BamHI ChlorR marker(1844,2503)<<< | | 1801 acgcatactgttatctggcttttagtaagccggatccacgcgattacgccccgccctgcc 1860 TGCGTATGACAATAGACCGAAAATCATTCGGCCTAGGTGCGCTAATGCGGGGCGGGACGG 1861 actcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaga 1920 TGAGTAGCGTCATGACAACATTAAGTAATTCGTAAGACGGCTGTACCTTCGGTAGTGTCT 1921 cggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatt 1980 GCCGTACTACTTGGACTTAGCGGTCGCCGTAGTCGTGGAACAGCGGAACGCATATTATAA NcoI | 1981 tgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaac 2040 ACGGGTACCACTTTTGCCCCCGCTTCTTCAACAGGTATAACCGGTGCAAATTTAGTTTTG 2041 tggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttag 2100 ACCACTTTGAGTGGGTCCCTAACCGACTCTGCTTTTTGTATAAGAGTTATTTGGGAAATC 2101 ggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaact 2160 CCTTTATCCGGTCCAAAAGTGGCATTGTGCGGTGTAGAACGCTTATATACACATCTTTGA 2161 gccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatgga 2220 CGGCCTTTAGCAGCACCATAAGTGAGGTCTCGCTACTTTTGCAAAGTCAAACGAGTACCT 2221 aaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgcca 2280 TTTGCCACATTGTTCCCACTTGTGATAGGGTATAGTGGTCGAGTGGCAGAAAGTAACGGT EcoRI | 2281 tacggaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaa 2340 ATGCCTTAAGGCCTACTCGTAAGTAGTCCGCCCGTTCTTACACTTATTTCCGGCCTATTT 2341 acttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtct 2400 TGAACACGAATAAAAAGAAATGCCAGAAATTTTTCCGGCATTATAGGTCGACTTGCCAGA 2401 ggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccatt 2460 CCAATATCCATGTAACTCGTTGACTGACTTTACGGAGTTTTACAAGAAATGCTACGGTAA 2461 gggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctc 2520 CCCTATATAGTTGCCACCATATAGGTCACTAAAAAAAGAGGTAAAATCGAAGGAATCGAG 2521 ctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaa 2580 GACTTTTAGAGCTATTGAGTTTTTTATGCGGGCCATCACTAGAATAAAGTAATACCACTT 2581 agttggaacctcttacgtgccgatcaacgtctcattttcgccaaaagttggcccagggct 2640 TCAACCTTGGAGAATGCACGGCTAGTTGCAGAGTAAAAGCGGTTTTCAACCGGGTCCCGA 2641 tcccggtatcaacagggacaccaggatttatttattctgcgaagtgatcttccgtcacag 2700 AGGGCCATAGTTGTCCCTGTGGTCCTAAATAAATAAGACGCTTCACTAGAAGGCAGTGTC attP other(2751,2982)<<< | 2701 gtatttattcggcgcaaagtgcgtcgggtgatgctgccaacttagtcgactacaggtcac 2760 CATAAATAAGCCGCGTTTCACGCAGCCCACTACGACGGTTGAATCAGCTGATGTCCAGTG 2761 taataccatctaagtagttgattcatagtgactggatatgttgtgttttacagtattatg 2820 ATTATGGTAGATTCATCAACTAAGTATCACTGACCTATACAACACAAAATGTCATAATAC 2821 tagtctgttttttatgcaaaatctaatttaatatattgatatttatatcattttacgttt 2880 ATCAGACAAAAAATACGTTTTAGATTAAATTATATAACTATAAATATAGTAAAATGCAAA 2881 ctcgttcagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgtt 2940 GAGCAAGTCGAAAGAACATGTTTCAACCGTAATATTCTTTCGTAACGAATAGTTAAACAA EcoRV | 2941 gcaacgaacaggtcactatcagtcaaaataaaatcattatttgccatccagctgatatcc 3000 CGTTGCTTGTCCAGTGATAGTCAGTTTTATTTTAGTAATAAACGGTAGGTCGACTATAGG T7 prom(3000,3019)<<< | 3001 cctatagtgagtcgtattacatggtcatagctgtttcctggcagctctggcccgtgtctc 3060 GGATATCACTCAGCATAATGTACCAGTATCGACAAAGGACCGTCGAGACCGGGCACAGAG XbaI | 3061 aaaatctctgatgttacattgcacaagataaaaatatatcatcatgcctcctctagacca 3120 TTTTAGAGACTACAATGTAACGTGTTCTATTTTTATATAGTAGTACGGAGGAGATCTGGT 3121 gccaggacagaaatgcctcgacttcgctgctgcccaaggttgccgggtgacgcacaccgt 3180 CGGTCCTGTCTTTACGGAGCTGAAGCGACGACGGGTTCCAACGGCCCACTGCGTGTGGCA 3181 ggaaacggatgaaggcacgaacccagtggacataagcctgttcggttcgtaagctgtaat 3240 CCTTTGCCTACTTCCGTGCTTGGGTCACCTGTATTCGGACAAGCCAAGCATTCGACATTA SpectR marker(3252,4262)>>> | 3241 gcaagtagcgtatgcgctcacgcaactggtccagaaccttgaccgaacgcagcggtggta 3300 CGTTCATCGCATACGCGAGTGCGTTGACCAGGTCTTGGAACTGGCTTGCGTCGCCACCAT 3301 acggcgcagtggcggttttcatggcttgttatgactgtttttttggggtacagtctatgc 3360 TGCCGCGTCACCGCCAAAAGTACCGAACAATACTGACAAAAAAACCCCATGTCAGATACG 3361 ctcgggcatccaagcagcaagcgcgttacgccgtgggtcgatgtttgatgttatggagca 3420 GAGCCCGTAGGTTCGTCGTTCGCGCAATGCGGCACCCAGCTACAAACTACAATACCTCGT 3421 gcaacgatgttacgcagcagggcagtcgccctaaaacaaagttaaacatcatgagggaag 3480 CGTTGCTACAATGCGTCGTCCCGTCAGCGGGATTTTGTTTCAATTTGTAGTACTCCCTTC 3481 cggtgatcgccgaagtatcgactcaactatcagaggtagttggcgtcatcgagcgccatc 3540 GCCACTAGCGGCTTCATAGCTGAGTTGATAGTCTCCATCAACCGCAGTAGCTCGCGGTAG 3541 tcgaaccgacgttgctggccgtacatttgtacggctccgcagtggatggcggcctgaagc 3600 AGCTTGGCTGCAACGACCGGCATGTAAACATGCCGAGGCGTCACCTACCGCCGGACTTCG 3601 cacacagtgatattgatttgctggttacggtgaccgtaaggcttgatgaaacaacgcggc 3660 GTGTGTCACTATAACTAAACGACCAATGCCACTGGCATTCCGAACTACTTTGTTGCGCCG BclI | 3661 gagctttgatcaacgaccttttggaaacttcggcttcccctggagagagcgagattctcc 3720 CTCGAAACTAGTTGCTGGAAAACCTTTGAAGCCGAAGGGGACCTCTCTCGCTCTAAGAGG 3721 gcgctgtagaagtcaccattgttgtgcacgacgacatcattccgtggcgttatccagcta 3780 CGCGACATCTTCAGTGGTAACAACACGTGCTGCTGTAGTAAGGCACCGCAATAGGTCGAT 3781 agcgcgaactgcaatttggagaatggcagcgcaatgacattcttgcaggtatcttcgagc 3840 TCGCGCTTGACGTTAAACCTCTTACCGTCGCGTTACTGTAAGAACGTCCATAGAAGCTCG 3841 cagccacgatcgacattgatctggctatcttgctgacaaaagcaagagaacatagcgttg 3900 GTCGGTGCTAGCTGTAACTAGACCGATAGAACGACTGTTTTCGTTCTCTTGTATCGCAAC 3901 ccttggtaggtccagcggcggaggaactctttgatccggttcctgaacaggatctatttg 3960 GGAACCATCCAGGTCGCCGCCTCCTTGAGAAACTAGGCCAAGGACTTGTCCTAGATAAAC 3961 aggcgctaaatgaaaccttaacgctatggaactcgccgcccgactgggctggcgatgagc 4020 TCCGCGATTTACTTTGGAATTGCGATACCTTGAGCGGCGGGCTGACCCGACCGCTACTCG 4021 gaaatgtagtgcttacgttgtcccgcatttggtacagcgcagtaaccggcaaaatcgcgc 4080 CTTTACATCACGAATGCAACAGGGCGTAAACCATGTCGCGTCATTGGCCGTTTTAGCGCG BglI | 4081 cgaaggatgtcgctgccgactgggcaatggagcgcctgccggcccagtatcagcccgtca 4140 GCTTCCTACAGCGACGGCTGACCCGTTACCTCGCGGACGGCCGGGTCATAGTCGGGCAGT 4141 tacttgaagctagacaggcttatcttggacaagaagaagatcgcttggcctcgcgcgcag 4200 ATGAACTTCGATCTGTCCGAATAGAACCTGTTCTTCTTCTAGCGAACCGGAGCGCGCGTC 4201 atcagttggaagaatttgtccactacgtgaaaggcgagatcaccaaggtagtcggcaaat 4260 TAGTCAACCTTCTTAAACAGGTGATGCACTTTCCGCTCTAGTGGTTCCATCAGCCGTTTA XhoI | 4261 aaccctcgagccacccatgaccaaaatcccttaacgtgagttacgcgtcgttccactgag 4320 TTGGGAGCTCGGTGGGTACTGGTTTTAGGGAATTGCACTCAATGCGCAGCAAGGTGACTC pUC origin(4339,4958)>>> | 4321 cgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaa 4380 GCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTCTAGGAAAAAAAGACGCGCATT 4381 tctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaag 4440 AGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTC 4441 agctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactg 4500 TCGATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGAC 4501 tccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacat 4560 AGGAAGATCACATCGGCATCAATCCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATGTA 4561 acctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtctta 4620 TGGAGCGAGACGATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAAT 4621 ccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggg 4680 GGCCCAACCTGAGTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCCCC 4681 gttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagc 4740 CAAGCACGTGTGTCGGGTCGAACCTCGCTTGCTGGATGTGGCTTGACTCTATGGATGTCG 4741 gtgagcattgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaa 4800 CACTCGTAACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCATT 4801 gcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatc 4860 CGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAG 4861 tttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgt 4920 AAATATCAGGACAGCCCAAAGCGGTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCA 4921 caggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggcct 4980 GTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGGA 4981 tttgctggccttttgctcacatgtt 5005 AAACGACCGGAAAACGAGTGTACAA