LOCUS pLOC 9192 bp ds-DNA circular 01-JUN-2020 DEFINITION Precision LentiORF general structure. Ns represent the ORF location and variations on the linkers between the Gateway sites. ACCESSION urn.local...1377636669111.8 KEYWORDS . SOURCE null ORGANISM . COMMENT . FEATURES Location/Qualifiers LTR 1..635 /label="5'-LTR" misc_binding 685..822 /label="Psi" misc_binding 1252..1605 /label="RRE" misc_binding 2028..2144 /label="cPPT/CTS" promoter 2201..2727 /label="CMV promoter" attB1 2863..2884 misc_feature 2887..2889 /label="variable" ORF 2890..2910 misc_feature 2911..2913 /label="variable" attB2 complement(2914..2935) misc_signal 2963..3531 /label="IRES" CDS 3538..>4290 /label="TurboGFP" CDS 4291..>4350 /label="FMDV 2A" CDS 4351..4791 /label="Blasticidin resistance" misc_binding 4805..5396 /label="WPRE" LTR 5601..5835 /label="3'-SIN-LTR" polyA_site 5942..6138 /label="bGH polyA" rep_origin 6211..6639 /label="F1 ori" promoter 6640..7023 /label="SV40 promoter" rep_origin 6833..6968 /label="SV40 origin" rep_origin complement(7060..7783) /label="pUC origin" CDS complement(7878..8738) /label="AmpR" promoter complement(8739..8843) /label="bla promoter" misc_feature 8891..9021 /label="SV40 polyA" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagatta attaagttta aacttaagct tggtaccgag 2821 ctcggatcca ctagtccagt gtggtggaat tctgcagata tcacaagttt gtacaaaaaa 2881 gywggcacca tgnnnnnnnn nnnnnnnnnn tdsccarctt tcttgtacaa agtggttgct 2941 agctaatgaa ccgggcgcgc cgcccctctc cctccccccc ccctaacgtt actggccgaa 3001 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt 3061 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg 3121 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc 3181 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc 3241 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa 3301 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc 3361 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg 3421 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac 3481 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga taataccatg 3541 cccaagaaga agagaaaggt ggagagcgac gagagcggcc tgcccgccat ggagatcgag 3601 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc 3661 acccccgagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc 3721 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc 3781 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc 3841 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc 3901 ggccgcgtga tcggcgactt caaggtgatg ggcaccggct tccccgagga cagcgtgatc 3961 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat 4021 aacgatctgg atggcagctt cacccgcacc ttcagcctgc gcgacggcgg ctactacagc 4081 tccgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac 4141 gggggcccca tgttcgcctt ccgccgcgtg gaggaggatc acagcaacac cgagctgggc 4201 atcgtggagt accagcacgc cttcaagacc ccggatgcag atgccggtga agaaagatct 4261 cgagctcaag cttcgaattc tgcagtcgag cagctgttga attttgacct tcttaagctt 4321 gcgggagacg tcgagtccaa ccctgggccc ggtaccagga gggccaccat gaagaccttc 4381 aacatctctc agcaggatct ggagctggtg gaggtcgcca ctgagaagat caccatgctc 4441 tatgaggaca acaagcacca tgtcggggcg gccatcagga ccaagactgg ggagatcatc 4501 tctgctgtcc acattgaggc ctacattggc agggtcactg tctgtgctga agccattgcc 4561 attgggtctg ctgtgagcaa cgggcagaag gactttgaca ccattgtggc tgtcaggcac 4621 ccctactctg atgaggtgga cagatccatc agggtggtca gcccctgtgg catgtgcaga 4681 gagctgatct ctgactatgc tcctgactgc tttgtgctca ttgagatgaa tggcaagctg 4741 gtcaaaacca ccattgagga actcatcccc ctcaagtaca ccaggaacta ataataagtt 4801 aaccaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 4861 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 4921 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 4981 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 5041 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 5101 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 5161 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct 5221 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 5281 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 5341 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat 5401 taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct 5461 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg tttttccagt 5521 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 5581 tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatctgct 5641 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 5701 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 5761 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 5821 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 5881 gaaatgaata tcagagagtg agaggccttg acattgttta aacccgctga tcagcctcga 5941 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc 6001 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc 6061 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt 6121 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa 6181 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg 6241 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 6301 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 6361 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 6421 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 6481 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 6541 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt 6601 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca 6661 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 6721 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 6781 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 6841 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 6901 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt 6961 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga 7021 tcagcacgtg atgaaaaagc ctgaactcac cgcgacgtca tgtgagcaaa aggccagcaa 7081 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 7141 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 7201 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 7261 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 7321 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 7381 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 7441 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 7501 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 7561 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 7621 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 7681 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 7741 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 7801 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 7861 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 7921 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 7981 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 8041 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 8101 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 8161 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 8221 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 8281 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 8341 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 8401 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 8461 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 8521 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 8581 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 8641 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 8701 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 8761 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 8821 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga 8881 tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 8941 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 9001 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa 9061 acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac 9121 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac 9181 aaacttagta gt //