LOCUS pLX_TRC304 9377 bp ds-DNA circular 16-OCT-2012 DEFINITION pLKO.1 TRC v1 library vector, with no insert. ACCESSION urn.local...1350417482037.7 VERSION KEYWORDS . SOURCE ORGANISM . COMMENT ApEinfo:methylated:1. FEATURES Location/Qualifiers CDS 8753..9166 /invitrogen=1050000 /vntifkey=4 /ApEinfo_fwdcolor="#00ff40" /ApEinfo_revcolor="#ff80ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 10 offset 0" /label="Bsd(R)" LTR 6528..6940 /vntifkey=19 /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="5'LTR" LTR 3043..3278 /vntifkey=19 /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="3'\LTR\SIN" promoter 7..588 /vntifkey=29 /ApEinfo_fwdcolor="#0000ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="CMV" promoter 8241..8743 /vntifkey=29 /ApEinfo_fwdcolor="#0000ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="PGK" primer_bind 471..490 /vntifkey=28 /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="pLEx-ORF-fwd" primer_bind 2751..2770 /vntifkey=28 /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="pLEx-ORF-rev" primer_bind 2343..2351 /vntifkey=28 /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="stop" rep_origin complement(5565..6180) /vntifkey=33 /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="pUC\Origin" rep_origin 3832..4262 /vntifkey=33 /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="F1\ORI" rep_origin 3516..3593 /created_by="User" /modified_by="User" /label="SV40 ori" misc_feature 4633..5439 /vntifkey=21 /ApEinfo_fwdcolor="#8080ff" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="AMP\r" misc_feature 828..1487 /vntifkey=21 /ApEinfo_fwdcolor="#8080ff" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="CmR" misc_feature 2384..2972 /vntifkey=21 /ApEinfo_fwdcolor="#8080ff" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="WPRE" misc_feature 7095..7459 /vntifkey=21 /ApEinfo_fwdcolor="#8080ff" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="del(gag)" misc_feature 1829..2134 /vntifkey=21 /ApEinfo_fwdcolor="#8080ff" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="ccdB" misc_feature 7605..7846 /vntifkey=21 /ApEinfo_fwdcolor="#8080ff" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="RRE" misc_feature 6991..7128 /vntifkey=21 /ApEinfo_fwdcolor="#8080ff" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="psi" misc_feature 595..718 /vntifkey=21 /ApEinfo_fwdcolor="#8080ff" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="attR1" misc_feature 2175..2298 /vntifkey=21 /ApEinfo_fwdcolor="#8080ff" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="attR2" misc_feature 9208..9325 /vntifkey=21 /ApEinfo_fwdcolor="#8080ff" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="cPPT" misc_feature 2301..2342 /vntifkey=21 /ApEinfo_fwdcolor="#8080ff" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="V5" ORIGIN 1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt 61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt 601 ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag 661 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg 721 cggccgcatt aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga 781 ttttgagtta ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa 841 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat 901 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt 961 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc 1021 gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat 1081 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc 1141 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg 1201 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg 1261 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca 1321 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga 1381 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc 1441 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg 1501 gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa 1561 tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat 1621 tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc 1681 tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg 1741 aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct 1801 tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagaga 1861 gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg 1921 gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta 1981 cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt 2041 gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa 2101 aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca 2161 gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt 2221 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag 2281 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 2341 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta 2401 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg 2461 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc 2521 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca 2581 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac 2641 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact 2701 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc 2761 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg 2821 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc 2881 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac 2941 gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag 3001 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac 3061 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc 3121 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 3181 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 3241 ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat 3301 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg 3361 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 3421 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 3481 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt 3541 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 3601 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt 3661 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg 3721 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt 3781 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag 3841 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt 3901 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt 3961 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc 4021 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga 4081 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc 4141 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt 4201 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct 4261 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc 4321 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat 4381 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 4441 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt 4501 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt 4561 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc 4621 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta 4681 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac 4741 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa 4801 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg 4861 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc 4921 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg 4981 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta 5041 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg 5101 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg 5161 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc 5221 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt 5281 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt 5341 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 5401 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 5461 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 5521 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 5581 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag 5641 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 5701 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 5761 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 5821 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 5881 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 5941 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 6001 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 6061 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 6121 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga 6181 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg 6241 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 6301 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt 6361 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt 6421 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag 6481 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt 6541 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag 6601 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat 6661 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca 6721 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga 6781 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct 6841 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat 6901 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt 6961 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg 7021 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg 7081 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg 7141 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt 7201 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga 7261 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact 7321 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa 7381 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc 7441 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa 7501 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg 7561 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg 7621 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg 7681 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg 7741 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc 7801 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa 7861 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac 7921 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct 7981 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat 8041 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt 8101 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg 8161 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc 8221 tcccaacccc gaggggaccc ttgcgccttt tccaaggcag ccctgggttt gcgcagggac 8281 gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat 8341 tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg 8401 cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga 8461 cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag 8521 caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg 8581 ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg 8641 ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag 8701 tcggctccct cgttgaccga atcaccgacc tctctcccca gggggtacca ccatggccaa 8761 gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat 8821 ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt 8881 cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg 8941 cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa 9001 caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg 9061 gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt 9121 gctgccctct ggttatgtgt gggagggcct gcagctgcag tagtaagaat tctagatctt 9181 gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag 9241 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa 9301 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg 9361 gcgccggctc gaggggg //