lacZ-alhpa reporter(1,160)<<< | 1 ccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgct 60 GGTAAGCGGTAAGTCCGACGCGTTGACAACCCTTCCCGCTAGCCACGCCCGGAGAAGCGA 61 attacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagg 120 TAATGCGGTCGACCGCTTTCCCCCTACACGACGTTCCGCTAATTCAACCCATTGCGGTCC 121 gttttcccagtcacgacgttgtaaaacgacggccagtgaattgaatttaggtgacactat 180 CAAAAGGGTCAGTGCTGCAACATTTTGCTGCCGGTCACTTAACTTAAATCCACTGTGATA BamHI | SphI HindIII XbaI NotI | | | | | 181 agaagagctatgacgtcgcatgcacgcgtacgtaagcttggatcctctagagcggccgcc 240 TCTTCTCGATACTGCAGCGTACGTGCGCATGCATTCGAACCTAGGAGATCTCGCCGGCGG AvrI | AvaI | AccI SmaI | | | SacI XmaI EcoRI KpnI PstI | | | | | | | 241 gactagtgagctcgtcgacccgggaattccggaccggtacctgcaggcgtaccagctttc 300 CTGATCACTCGAGCAGCTGGGCCCTTAAGGCCTGGCCATGGACGTCCGCATGGTCGAAAG T7 prom(300,319)<<< lac prom(343,458)<<< | | 301 cctatagtgagtcgtattagagcttggcgtaatcatggtcatagctgtttcctgtgtgaa 360 GGATATCACTCAGCATAATCTCGAACCGCATTAGTACCAGTATCGACAAAGGACACACTT 361 attgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcct 420 TAACAATAGGCGAGTGTTAAGGTGTGTTGTATGCTCGGCCTTCGTATTTCACATTTCGGA lacI reg(465,1556)<<< | 421 ggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttcc 480 CCCCACGGATTACTCACTCGATTGAGTGTAATTAACGCAACGCGAGTGACGGGCGAAAGG 481 agtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcg 540 TCAGCCCTTTGGACAGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCCCCTCTCCGC NarI | 541 gtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctga 600 CAAACGCATAACCCGCGGTCCCACCAAAAAGAAAAGTGGTCACTCTGCCCGTTGTCGACT 601 ttgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgcccc 660 AACGGGAAGTGGCGGACCGGGACTCTCTCAACGTCGTTCGCCAGGTGCGACCAAACGGGG HpaI | 661 agcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcg 720 TCGTCCGCTTTTAGGACAAACTACCACCAATTGCCGCCCTATATTGTACTCGACAGAAGC EcoRV | 721 gtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatg 780 CATAGCAGCATAGGGTGATGGCTCTATAGGCGTGGTTGCGCGTCGGGCCTGAGCCATTAC 781 gcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatg 840 CGCGCGTAACGCGGGTCGCGGTAGACTAGCAACCGTTGGTCGTAGCGTCACCCTTGCTAC 841 ccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcc 900 GGGAGTAAGTCGTAAACGTACCAAACAACTTTTGGCCTGTACCGTGAGGTCAGCGGAAGG 901 cgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgc 960 GCAAGGCGATAGCCGACTTAAACTAACGCTCACTCTATAAATACGGTCGGTCGGTCTGCG ApaI | 961 agacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaat 1020 TCTGCGCGGCTCTGTCTTGAATTACCCGGGCGATTGTCGCGCTAAACGACCACTGGGTTA 1021 gcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttg 1080 CGCTGGTCTACGAGGTGCGGGTCAGCGCATGGCAGAAGTACCCTCTTTTATTATGACAAC 1081 atgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttcc 1140 TACCCACAGACCAGTCTCTGTAGTTCTTTATTGCGGCCTTGTAATCACGTCCGTCGAAGG BclI | 1141 acagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacccgttgc 1200 TGTCGTTACCGTAGGACCAGTAGGTCGCCTATCAATTACTAGTCGGGTGACTGGGCAACG 1201 gcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgac 1260 CGCTCTTCTAACACGTGGCGGCGAAATGTCCGAAGCTGCGGCGAAGCAAGATGGTAGCTG 1261 accaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgac 1320 TGGTGGTGCGACCGTGGGTCAACTAGCCGCGCTCTAAATTAGCGGCGCTGTTAAACGCTG 1321 ggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgcc 1380 CCGCGCACGTCCCGGTCTGACCTCCACCGTTGCGGTTAGTCGTTGCTGACAAACGGGCGG 1381 agttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttt 1440 TCAACAACACGGTGCGCCAACCCTTACATTAAGTCGAGGCGGTAGCGGCGAAGGTGAAAA 1441 tcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataa 1500 AGGGCGCAAAAGCGTCTTTGCACCGACCGGACCAAGTGGTGCGCCCTTTGCCAGACTATT lacI prom(1557,1635)<<< | 1501 gagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctg 1560 CTCTGTGGCCGTATGAGACGCTGTAGCATATTGCAATGACCAAAGTGTAAGTGGTGGGAC 1561 aattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatg 1620 TTAACTGAGAGAAGGCCCGCGATAGTACGGTATGGCGCTTTCCAAAACGCGGTAAGCTAC 1621 gtgtcaacgtaaatgccgcttcgccttcgcgcgcgaattgcaagctctgcattaatgaat 1680 CACAGTTGCATTTACGGCGAAGCGGAAGCGCGCGCTTAACGTTCGAGACGTAATTACTTA 1681 cggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcac 1740 GCCGGTTGCGCGCCCCTCTCCGCCAAACGCATAACCCGCGAGAAGGCGAAGGAGCGAGTG 1741 tgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggt 1800 ACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCA 1801 aatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggcca 1860 TTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGT pUC origin(1888,2507)<<< | 1861 gcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgccc 1920 CGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGG 1921 ccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggact 1980 GGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGA 1981 ataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccct 2040 TATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGA 2041 gccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcaatg 2100 CGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTTAC 2101 ctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgca 2160 GAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGT 2161 cgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaa 2220 GCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTT AlwNI | 2221 cccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagc 2280 GGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCG 2281 gaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactag 2340 CTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATC 2341 aaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttgg 2400 TTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACC 2401 tagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagca 2460 ATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGT 2461 gcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtc 2520 CGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAG 2521 tgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaag 2580 ACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTC 2581 gatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatata 2640 CTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATAT AmpR marker(2662,3522)<<< | 2641 tgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgat 2700 ACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTA 2701 ctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacg 2760 GACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGC 2761 ggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggc 2820 CCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCG 2821 tccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgc 2880 AGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACG 2881 aactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttc 2940 TTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAG 2941 gccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctc 3000 CGGTCAATTATCAAACGCGTTGCAACAACGGTAACGATGTCCGTAGCACCACAGTGCGAG 3001 gtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatc 3060 CAGCAAACCATACCGAAGTAAGTCGAGGCCAAGGGTTGCTAGTTCCGCTCAATGTACTAG 3061 ccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaa 3120 GGGGTACAACACGTTTTTTCGCCAATCGAGGAAGCCAGGAGGCTAGCAACAGTCTTCATT 3121 gttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcat 3180 CAACCGGCGTCACAATAGTGAGTACCAATACCGTCGTGACGTATTAAGAGAATGACAGTA 3181 gccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaata 3240 CGGTAGGCATTCTACGAAAAGACACTGACCACTCATGAGTTGGTTCAGTAAGACTCTTAT 3241 gtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccaca 3300 CACATACGCCGCTGGCTCAACGAGAACGGGCCGCAGTTATGCCCTATTATGGCGCGGTGT 3301 tagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaag 3360 ATCGTCTTGAAATTTTCACGAGTAGTAACCTTTTGCAAGAAGCCCCGCTTTTGAGAGTTC 3361 gatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttc 3420 CTAGAATGGCGACAACTCTAGGTCAAGCTACATTGGGTGAGCACGTGGGTTGACTAGAAG 3421 agcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgc 3480 TCGTAGAAAATGAAAGTGGTCGCAAAGACCCACTCGTTTTTGTCCTTCCGTTTTACGGCG 3481 aaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaata 3540 TTTTTTCCCTTATTCCCGCTGTGCCTTTACAACTTATGAGTATGAGAAGGAAAAAGTTAT Bacterial-Promoter prom(3564,3602)<<< | 3541 ttattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtattta 3600 AATAACTTCGTAAATAGTCCCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAAT 3601 gaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgaaattgt 3660 CTTTTTATTTGTTTATCCCCAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTTTAACA 3661 aaacgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttttaa 3720 TTTGCAATTATAAAACAATTTTAAGCGCAATTTAAAAACAATTTAGTCGAGTAAAAAATT 3721 ccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggtt 3780 GGTTATCCGGCTTTAGCCGTTTTAGGGAATATTTAGTTTTCTTATCTGGCTCTATCCCAA f1 origin(3786,4092)<<< | 3781 gagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaa 3840 CTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTT 3841 agggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaag 3900 TCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGATTAGTTC 3901 ttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatt 3960 AAAAAACCCCAGCTCCACGGCATTTCGTGATTTAGCCTTGGGATTTCCCTCGGGGGCTAA 3961 tagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaagg 4020 ATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCC 4021 agcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgc 4080 TCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCG 4081 cgcgcttaatgcgccgctacagggcgcgtc 4110 GCGCGAATTACGCGGCGATGTCCCGCGCAG