LOCUS       dna                      1690 bp                                   
FEATURES             Location/Qualifiers
     Promoter        4..23
                     /gene="T3 prom"
     misc_binding    53..60
                     /dbxref="REBASE:NotI"
     misc_binding    61..73
                     /dbxref="REBASE:SfiI"
     misc_binding    62..72
                     /dbxref="REBASE:BglI"
     misc_binding    74..79
                     /dbxref="REBASE:EcoRV"
     misc_binding    80..85
                     /dbxref="REBASE:EcoRI"
     misc_binding    80..85
                     /dbxref="REBASE:ApoI"
     misc_binding    94..99
                     /dbxref="REBASE:AccI"
     misc_binding    94..99
                     /dbxref="REBASE:HincII"
     Promoter        136..155
                     /gene="T7 prom"
     Rep_Origin      202..815
                     /gene="pUC origin"
     misc_binding    567..575
                     /dbxref="REBASE:AlwNI"
     Marker          814..1673
                     /gene="AmpR marker"
     misc_binding    1461..1466
                     /dbxref="REBASE:DraI"
BASE COUNT    412 a   456 c   406 g   416 t    0 others
ORIGIN
        1 attaattaac cctcactaaa gggacctaac tataacggtc ctaaggtagc gagcggccgc     
       61 ggccagggag gccgatatcg aattcggcgc gccgtcgact ttcattacct ctttctccgc     
      121 accccacata aatctcccta tagtgagtcg tattaacatg tgagcaaaag gccagcaaaa     
      181 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga     
      241 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag     
      301 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct     
      361 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg     
      421 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc     
      481 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt     
      541 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta     
      601 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac     
      661 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc     
      721 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat     
      781 tacgcgcaga aaaaaaggat ctcaagaaga tccttaccaa tgcttaatca gtgaggcacc     
      841 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat     
      901 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc     
      961 acgctcaccg gctccagatt tatcagcaat aaaccagcca gcggaagggc cgagcgcaga     
     1021 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga     
     1081 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg     
     1141 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga     
     1201 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt     
     1261 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct     
     1321 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca     
     1381 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat     
     1441 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga     
     1501 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc     
     1561 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg     
     1621 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc     
     1681 ctttttcaat                                                            
//