Polyclonal antibody affinity purified from goat serum immunized with purified rabbit IgG.
Antibodies labeled with a biotin analog have a seven atom linker consisting of 6-aminocaproic acid and the 6 atom side chain of biotin.
Contents:Each vial contains 0.5 mg of affinity purified antibody.
Labeling with alkaline phosphatase (AP) was carried out utilizing a modified glutaraldehyde procedure.
Contents: Each vial contains 1 mg of affinity purified antibody at 1 mg/mL. Supplied as liquid, stable for a minimum of 1 year at 2 - 8°C.
Labeling with horseradish peroxidase (HRP) was done using a periodate method.
Contents: Each vial contains 1 mg of affinity purified antibody at 1 mg/mL. The molar enzyme/antibody protein ratio is 3.0 for NEF811 and 4.0 for NEF812.
These products are tested by immnoelectrophoresis, gel diffusion and ELISA techniques. They react specifically with IgG and may recognize other immunoglobulin types that have light chains in common with IgG. Antibodies to IgG may cross react with the immunoglobulins of other mammalian species if common binding sites are shared. No antibody is detectable to non-immunoglobulin serum components.
Labeled affinity purified antibodies are excellent for both histochemical/cytochemical staining procedures and for use in enzyme amplified immunoassays such as microplate and membrane ELISA. Serial dilutions of all antibody reagents should be performed to determine the best working concentrations under a given set of assay conditions.