PhenoVue™ cell painting JUMP kit
PhenoVue™ Cell Painting JUMP Kits comprises 6 validated, pre-optimized fluorescent probes, in accordance with the JUMP consortium protocol v3, and associated diluent to streamline the cell painting workflow.
The kits offer excellent batch-to-batch reproducibility, are supplied with a step-by-step protocol, and are available in a range of pack sizes to match your throughput requirements.
PhenoVue Cell Painting JUMP kits are validated for use on high-content screening systems such as the Opera Phenix® Plus system.
Cell Painting is a powerful phenotypic high-content screening approach which combines cell and computational biology to unravel cells’ responses when subjected to perturbagens.
Cell are "painted" by labelling different cellular compartments with different fluorescent bioprobes to quantitatively profile multiple phenotypic parameters in order to better understand the effects of chemical compounds, drugs, genes, or other test articles.
PhenoVue Cell Painting JUMP Kits comprise validated, pre-optimized fluorescent bioprobes, aligned to the JUMP consortium protocol v3, to streamline your workflow, saving time and costs.
PhenoVue Cell Painting JUMP Kits provide:
- Excellent batch-to-batch reproducibility
The cell painting kit reagents are tested extensively (>80 parameters) using state-of-the-art techniques to guarantee high quality e.g. LCMS, HPLC, absorbance spectra, concentration, high-content imaging, degree of labelling (DoL), with strict acceptance criteria to deliver reliable and accurate results. - Ease-of-use
The reagents in the cell painting kits are supplied in optimized formulations to facilitate assay set-up, together with a detailed step-by-step protocol. - Convenient pack sizes
The cell painting kits are available in three different sizes, 1 x 384-well plate, 10 x 384-well plates and 100 x 384-well plates to meet your throughput needs and avoid waste. - Tried and Trusted
Used by the Joint Undertaking in Morphological Profiling-Cell Painting (JUMP-CP) consortium in creating the world’s largest, public cell painting dataset. Learn more here: https://ir.perkinelmer.com/news-releases/news-release-details/perkinelmer-supports-jump-cp-consortium-creating-worlds-largest
Kit Components / Quantity
- PhenoVue Hoechst 33342 Nuclear Stain / 1 vial - 70µg
- PhenoVue Fluor 488 - Concanavalin A / 1 vial - 1.2mg
- PhenoVue 512 Nucleic Acid Stain / 1 vial - 100nmol
- PhenoVue Fluor 555 - WGA / 1 vial - 0.02mg
- PhenoVue Fluor 568 - Phalloidin / 1 vial - 0.4nmol
- PhenoVue 641 Mitochondrial Stain / 1 vial - 22µg
- PhenoVue Dye Diluent A (5X) / 1 vial - 8mL (5X)
