PhenoVue™ Fluor - WGA is a fluorescent dye conjugated lectin which displays high affinity for sialic acid and N-acetylglucosamine residues of glycoproteins and glycolipids present at the cellular plasma membranes. It can be used for cellular membrane staining, particularly the Golgi apparatus, and is validated for use in imaging microscopy and high-content screening applications.
Fluorescent dye options include PhenoVue 488 (bright green) and PhenoVue 594 (bright red).
WGA is a plant lectin that binds different carbohydrate motifs and displays high affinity for sialic acid and N-acetylglucosamine residues of glycoproteins and glycolipids present at the cellular plasma membranes.
Fluorescent WGA derivatives are commonly used for staining the cellular membranes of mammalian cells, particularly Golgi apparatus which is glycoprotein-enriched. In addition, fluorescent WGA derivatives are also routinely used for the staining of skeletal muscle cells and tissues, as well as in cardiac fibrosis.
PhenoVue Fluor 488 or Fluor 594 - WGA can be used to visualize cellular membranes in immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
HeLa cells stained with PhenoVue Fluor 594 – WGA
Vials per unit
Volume per vial
1 mg (29.2 nmol)
Recommended working concentration
5 µg/mL (146nM)
Max Ex/Em wavelengths (nm)
495/520 (Fluor 488) 590/617 (Fluor 594)
Common filter set
FITC (Fluor 488)
Texas Red (Fluor 594)
Live or fixed*
Equivalent number of microplates
36-100 x 96-well microplates
30-100 x 384-well microplates
55-100 x 1536-well microplates