- Downstream analysis reagents & consumables
- PhenoVue cellular imaging reagents
- PhenoVue cytoskeleton/actin stain (Phalloidin)
PhenoVue™ cytoskeleton/actin stain (Phalloidin)
PhenoVue™ Fluor - Phalloidin is a fluorescent dye conjugated bicyclic peptide which binds specifically to F-actin and stains the actin cytoskeleton, and is validated for use in imaging microscopy and high-content screening applications.
Fluorescent dye options include PhenoVue 488 (bright green), PhenoVue 568 (bright red-orange), and PhenoVue 594 (bright red).
Phalloidin is a toxin isolated from the fungus Amanita phalloides. It is a bicyclic peptide that specifically binds and stabilizes actin filaments.
Fluorescent Phalloidin derivatives have been extensively used for monitoring actin expression and dynamics of the cytoskeleton network. The study of actin cytoskeleton regulation is of interest in the context of several pathophysiological processes such as cancer and neurodegenerative diseases as well as fibrosis and vascular diseases.
PhenoVue Fluor - Phalloidin can be used to vizualize actin filaments in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
HeLa cells stained with PhenoVue Fluor 488 – Phalloidin
HeLa cells stained with PhenoVue Fluor 488 – Phalloidin
HeLa cell stained with PhenoVue Fluor 568
HeLa cell stained with PhenoVue Fluor 568
HeLa cell stained with PhenoVue Fluor 594 - Phalloidon
HeLa cell stained with PhenoVue Fluor 594 - Phalloidon
Vials per unit | 1 |
Volume per vial | 300 units (10nmoles; 13µg) |
Form | Lyophilized |
Storage | 2-8°C |
Recommended working concentration | 5 µg/mL (146nM) |
Max Ex/Em wavelengths (nm) | 495/520 (Fluor 488) 578/603 (Fluor 560) 590/617 (Fluor 594) |
Common filter set | FITC (Fluor 488) Texas Red (Fluor 560) Texas Red (Fluor 594) |
Cell staining | Fixed |
Equivalent number of microplates | 2-6 x 96-well microplates 1.5-6 x 384-well microplates 3-10 x 1536-well microplates |
Application notes
Safety data sheets
Technical notes
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