- Downstream analysis reagents & consumables
- PhenoVue cellular imaging reagents
- PhenoVue lipid stain
PhenoVue™ lipid stain
The PhenoVue™ lipid stain is a fluorescent dye conjugated lipophilic organic molecule commonly used for localization and quantification of intracellular lipid droplets, and is validated for use in imaging microscopy and high-content screening applications.
Fluorescent dye options include PhenoVue 493 (bright green alternative to BODIPY™ 493/503) and PhenoVue Nile Red (bright yellow).
PhenoVue Nile Red Lipid stain is a lipophilic organic molecule that is almost non-fluorescent in water and polar solvents. In lipid-rich environments, Nile Red exhibits enhanced yellow fluorescence, as well as red fluorescence to a lesser extent.
PhenoVue 493 Lipid stain is a lipophilic fluorescent organic molecule that localizes to polar lipids. It rapidly accumulates in lipid droplets and exhibits enhanced green fluorescence in the presence of neutral lipids like triglycerides and cholesterol esters.
These lipid stains are commonly used for localization and quantification of intracellular lipid droplets which are involved in lipid synthesis, metabolism and transportation as well as protein storage and degradation or viral replication, all of which are related to pathophysiology, including dyslipidemia, obesity, lipodystrophy, diabetes, fatty liver diseases, or atherosclerosis.
HeLa cells stained with PhenoVue Nile-Red
HeLa cells stained with PhenoVue 493 Lipid stain
PhenoVue™ Fluor 493
Vials per unit | 5 |
Volume per vial | 2mg (4mM-1.05g/L) |
Form | Liquid |
Storage | -20°C |
Recommended working concentration | 5µM (1.31mg/L) |
Max Ex/Em wavelengths (nm) |
493/504 |
Common filter set | FITC |
Cell staining | Live or fixed* |
Equivalent number of microplates | 270-830 x 96-well microplates 230-830 x 384-well microplates 430-1300 x 1536-well microplates |
*see product information sheet for more details
PhenoVue™ Nile Red
Vials per unit | 2 |
Volume per vial | 10mg (31.4µmoles) |
Form | Lyophilized |
Storage | -20°C |
Recommended working concentration | 200nM (0.008ng/mL) |
Max Ex/Em wavelengths (nm) |
512/585 - when complexed with triglycerides |
Common filter set | Cy3.5 |
Cell staining | Live or fixed* |
Equivalent number of microplates | 10900-32700 x 96-well microplates 9090-32700 x 384-well microplates 17040-40900 x 1536-well microplates |
*see product information sheet for more details
Application notes
Safety data sheets
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