PhenoVue™ fluorescent secondary antibodies can be used in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
Goat anti-IgG (H+L) antibodies recognize IgG1, IgG2a, IgG2b and IgG3 isotypes. All antibodies are affinity purified.
Fluorescent label options include PhenoVue 488 (bright green), PhenoVue 568 (bright red-orange) and PhenoVue 594 (bright red).
- Cross-adsorbed antibodies may display cross-reactivity with other species. For improved specificity and lower background, or in multiplexed applications, consider using highly cross-adsorbed PhenoVue conjugated secondary antibodies as an alternative.
- Highly cross-adsorbed antibodies have been cross-adsorbed against various IgG species such as rat, hamster, human, rabbit, bovine, horse, or chicken serum to minimize cross-reactivity with other species.
|Vials per unit||1|
|Volume per vial||1 mg|
|Recommended working concentration||1.5 µg/mL (10nM)
|Max Ex/Em wavelengths (nm)||
495/520 (Fluor 488)
|Common filter set||FITC (Fluor 488)
Texas Red (Fluor 568, Fluor 594)
|Cell staining||Fixed only|
|Equivalent number of microplates||25-70 x 96-well microplates
20-70 x 384-well microplates
35-90 x 1536-well microplates