ATPlite™ is an Adenosine TriPhosphate (ATP) monitoring system based on firefly (Photinus pyralis) luciferase. This luminescence assay is the alternative to colorimetric, fluorometric and radioisotopic assays for the quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells or 3D spheroids. ATP monitoring can be used to assess the cytocidal, cytostatic and proliferative effects of a wide range of drugs, biological response modifiers and biological compounds.

ATPlite luminescence assay systems use patented innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the production of light caused by the reaction of ATP with added luciferase and D-luciferin.

ATPlite advantages

• Long-lived luminescent signal: half-life (t1/2) greater than 5 hours, depending on cell type and medium
• Rapid: results generated in 15 - 25 minutes (cultured cells) or 40 - 45 minutes (3D spheroids)
• Simple and reproducible: no separation steps; only two reagent additions
• Homogeneous assay: no cell harvesting or centrifugation required
• Sensitive: down to 5 cells in 100µL medium (derived from CHO and HL-60 cells in 100µL medium)
• Wide linear dynamic range: =10⁵ (as derived from CHO and HL-60 cells) or detect ATP from spheroids made by seeding anywhere from 200 - 5000 cells
• Convenient storage conditions (2 - 8 °C)
• No DTT: Unlike most luciferase detection reagents, lites products do not contain DTT, eliminating toxic hazards, unpleasant odors and the need for hood work
• Excellent Z' Values: All lites products offer robust, sensitive performance, with high signal-to-background ratios and excellent Z'values

An alternative format of this assay is also available. The ATPlite 1step assay has only one reagent addition step and no separation steps, and is designed for continuous processing workflows.