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Luminescence Assays

Luciferase assays allow for the study of transcriptional gene expression, virus life cycles, and cell viability, making them significant tools for drug development in reporter gene, cytotoxicity, and cell proliferation applications, as well as general kinase activity monitoring. Our luminescence assays include reporter gene, ATP-monitoring, and kinase assays.  

A luminescence assay is extremely useful as a detection platform for several reasons, including:

  • Wide dynamic range
  • Greater sensitivity than fluorescence technologies
  • Lower interference than other detection options
  • Cost effective detection
  • Simple automation for high throughput applications
  • Homogenous luciferase assay set-up eliminates wash and separation steps

Our luciferase luminescence assay options allow high sensitivity in a convenient microplate format.


For research use only. Not for use in diagnostic procedures. 

Luminescence Assays
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Reporter gene assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, Revvity provides a solution.

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, Revvity provides a solution.

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Cytotoxicity and cell proliferation

For fast, reliable detection of ATP for cell quantitation, our innovative luminescence technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

For fast, reliable detection of ATP for cell quantitation, our innovative luminescence technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

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Kinase activity

For fast, reliable detection of any kinase activity in a one-step, homogeneous assay format.

For fast, reliable detection of any kinase activity in a one-step, homogeneous assay format.

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easylite Kinase products
USD 142.34 - 528.85
ATPlite Luminescence Assay System, 1,000/4,000 image
ATPlite 1step Luminescence Assay System, 1,000 mL image
USD 87.28 - 4,334.00
Twinlite Glow assay kit 1,000 assay points image
USD 284.69 - 1,803.00
britelite plus Reporter Gene Assay System, 1,000 mL image
USD 117.41 - 6,962.00
neolite Reporter Gene Assay System, 1,000 mL image
USD 117.41 - 6,858.00
Shipping box for Revvity reagent kits
USD 203.64
Shipping box for Revvity reagent kits
USD 102.86
steadylite plus Reporter Gene Assay System, 1,000 mL image
USD 102.86 - 6,546.00
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Application Note
ATPlite assay performance in human primary cells.

In this application note three ATP monitoring luminescence assays were assessed and compared head-to-head: (i) ATPlite™ from...

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Comparison of EMT Biomarker Expression in 2D Monolayer and 3D Spheroid Cultures in a Prostate Cancer

AlphaLISA and LANCE (TR-FRET) biomarker assays can be used to measure ECM-associated protein modulation caused by human...

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Multi-parametric high-throughput analysis of hepatotoxicity using a 3D primary liver cell culture model

3D cell cultures offer a more physiologically relevant option, but there are currently only a few high throughput screening (HTS)...

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Proliferation and cell death analyses of 3D cultures using Revvity CellCarrier Spheroid ULA microplates and ATPlite 3D products.

In the process of developing new therapeutic molecules, toxicity – whether it is to be avoided or whether it is desired – is an...

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Rapid, No-wash Measurement of Immune Checkpoint Molecules and Cytokines in Co-cultures of Immune Cells and Cancer Cell Lines

Breast cancer tumors can adapt to immune cell infiltration by responding to the increased concentration of interferon gamma (IFN-ɣ...

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