Tagged Organelle Cell Lines

Our ready-made tagged organelle cell lines enable real time tracking of protein activity in live cells. These are a great alternative to unreliable antibodies, and are ideal markers to determine:

  • The localization of a particular protein or organelle
  • Protein interactions
  • Protein concentration

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How do endogenous markers compare to:

Advantages

Vectors

No molecular biology capabilities are required

Overexpression systems

Protein expression is at the endogenous level, so these tagged cell lines avoid problems with artifacts in fluorescence microscopy, such as ectopic sub-cellular localizations or erroneous formation of protein complexes

Conventional gene tagging

Every copy of the gene is tagged making quantification simpler. In conventional methods, untagged versions of a protein may be present.

Antibody

Relatively little preparation is required to use the endogenously tagged cell lines, and these are compatible with live cell assays. Unlike antibodies there are no concerns over specificity.

Organelle specific dyes

Endogenously tagged cell lines contain an early maturation fluorescence protein allowing for early detection, and so are not dependent on specific organelle conditions for activity.

Examples of Fluorescent Markers for Cell Organelles

calr-gene-turbo-gfp-tagged-endoplasmic-reticulumOrganelle: Endoplasmic Reticulum
Gene tagged: CALR
Protein encoded: Calreticulin
Function: Calreticulin is localized to storage compartments associated with the endoplasmic reticulum

pdia4-gene-turbogfp-tagged-endoplasmic-reticulumOrganelle: Endoplasmic Reticulum
Gene tagged: PDIA4
Protein: Protein Disulfide Isomerase Family A Member 4
Function: PDIA4 is an endoplasmic reticulum luminal protein. This enzyme catalyzes the formation and breakage of disulfide bonds during protein folding.

pcm1-gene-turbogfp-tagged-centrisomeOrganelle: Centrosomes
Gene tagged: PCM1
Protein: Pericentriolar Material 1
Function: The PCM1 gene encodes a component of centriolar satellites, localized adjacent to centrosomes, and plays a pivotal roles in centrosome assembly.

h3f3b-gene-turbogfp-tagged-nucleusOrganelle: Nucleus
Gene tagged: H3F3B
Protein: H3 Histone, Family 3B (H3.3B)
Function: Histones are small basic proteins that package and organize DNA to form nucleosome particles.

top2a-gene-turbo-gfp-tagged-nucleusOrganelle: Nucleus
Gene tagged: TOP2A
Protein: DNA topoisomerase
Function: This nuclear enzyme controls the topologic states of DNA during transcription, in processes such as chromosome condensation and chromatid separation.

hk1-gene-turbogfp-tagged-mitochondriaOrgenelle: Mitochondria
Gene tagged: HK1
Protein: Hexokinase 1
Function: This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria.

Organelle tagged cell lines

Location

Protein

Gene

Actin

Beta Actin

ACTB

Centrosomes

Pericentriolar Material 1

PCM1

Endosomes

Early Endosome Antigen 1

EEA1

ER

Calreticulin

CALR

ER

Protein Disulfide Isomerase Family A Member 4

PDIA4

Mitochondria

Hexokinase 1

HK1

Nucleus

H3 Histone, Family 3B (H3.3B)

H3F3B

Peroxisomes

Catalase

CAT

Plasma membrane, Cell junctions

Cadherin 2

CDH2

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