Ideal for use in primary or difficult to transfect cells, Precision LentiORFs can be used to create stably expressing cell lines, investigate overexpression phenotypes, or perform RNAi rescue experiments.

Highlights

• Lentiviral ORF constructs require no subcloning for mammalian expression
• Fully sequenced coding sequences
• CMV promoter for robust protein expression
• GFP reporter is transported to the nucleus for easy tracking of protein expression
• Blasticidin resistance for selection of stable cell lines
• Secondary tags can be added to the MultiTag cloning site located downstream of the coding sequence
• Clones are available with or without native stop codons

Formats

• Individual Clones – Glycerol Stock
• Pre-defined gene families –Arrayed glycerol stocks (with or without native stop clones)
• Complete genome-scale collection –Arrayed glycerol stocks(with or without native stop clones)
• Viral Particles – provided as 2x25µL aliquots with a minimum titer of 10^8TU/mL
• LentiORF Starter Kits – Choose from transfection kits, transduction kits, and viral particle kits

Applications

Potential applications of Precision LentiORFs include gene overexpression experiments, RNAi rescue (see the RNAi Rescue using Precision LentiORF technical note), and expression of human proteins in mammalian cell lines. Primary and non-dividing cells can be readily transduced with ready-to-use lentiviral particles. Alternatively, Precision LentiORF constructs can be transfected into your cell line. For generating your own viral particles, Trans-Lentiviral ORF packaging kits are also available.

Precision LentiORF Viral Particle starter kits include:

• Two Precision LentiORF constructs provided as 2x25µL aliquots of high-titer viral particles (1x10^8TU/mL)
• Precision LentiORF RFP control provided as 25µL of high-titer virus

Precision LentiORF Transduction starter kits include:

• Two Precision LentiORF constructs
• Trans-Lentiviral packaging mix sufficient for 10 packaging events
• Calcium Phosphate transfection reagent
• Precision LentiORF RFP control

Note

The Precision LentiORF expression vectors are not compatible with third generation packaging systems. We recommend the Trans-Lentiviral Packaging System for use with these vectors.

Individual Clones

Clones are provided as a live culture in a 2mL tube. Each tube contains LB medium supplemented with 8% glycerol and the appropriate antibiotic. Within 2 to 3 business days of receiving your order we will ship your clone at room temperature via express delivery. Store the stock clone at –80°C.

Clone Collections

Orders of 50 clones or greater and stock plates will be provided in 96-well microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at –80°C immediately upon receipt. Please contact our customer service department for a quote and estimated shipping time on bulk orders.

Viral Particles

Due to the inherent complexity of high-titer lentiviral particle production, we estimate a 6 to 8 week turnaround time for these orders.

Disclaimer

Dharmacon is a distributor of multiple gene expression clone collections (cDNAs and ORFs). These clone collections were generated by groups outside of Dharmacon and thus the quality of the collections is largely dependent upon what Dharmacon received from these groups. Specific clone information and plate coordinates were provided to Dharmacon by the suppliers of these clone collections. Dharmacon has not sequence verified each individual clone from these collections. These collections and individual clones are distributed "as is" with no additional product validation or guarantees. Dharmacon has established quality procedures to ensure that individual clones are picked from the identified well in a plate, grown on the correct antibiotic, and are free of phage contamination. Due to the quality of the information provided to Dharmacon, the clone you receive might not match the expected clone. If this occurs, please contact Scientific Support Team.