Cas9 nuclease protein NLS
A DNA-free option for Cas9 nuclease expression
Purified Cas9 nuclease protein NLS for co-transfection with synthetic guide RNA for a completely DNA-free genome engineering system
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*Note: Concentration and pack sizes have been updated and are reflected with new catalog numbers
Cas9 nuclease in the CRISPR-Cas9 system
The CRISPR-associated enzyme Cas9 is an RNA-guided endonuclease that requires a guide RNA for genomic DNA target recognition and generation of DNA double-strand breaks (DSB).
Purified Cas9 nuclease NLS protein offers a ready-to-use option, enabling researchers to accelerate genome engineering experiments in a completely DNA-free manner.
Cas9 protein highlights
- Straightforward co-transfection or co-electroporation with synthetic guide RNA
- No external DNA added to system to ensure against the possibility of incorporating plasmid DNA into the host cell's genome
- No issues with incompatibilities between promoter and cell line
- Transient expression of Cas9 nuclease to reduce off targeting
DharmaFECT transfection reagents are highly recommended for use with Edit-R™ gene editing reagents and should be purchased separately. Refer to the DharmaFECT Cell Type Guide to find the appropriate formulation for your cell type.
Quantity & concentration of Cas9 nuclease protein NLS reagents
| Catalog # | Concentration | Volume | Quantity | ||
|---|---|---|---|---|---|
| µM | µg/µl | µl | µg | pMol | |
| CAS12205 | 61.8 | 10 | 10 | 100 | 618 |
| CAS12206 | 61.8 | 10 | 50 | 500 | 3090 |
| CAS12207 | 61.8 | 10 | 5 x 50 | 5 x 500 | 5 x 3090 |
| CAS11728 (discontinued) | 20 | 3.2 | 12.5 | 40 | 250 |
| CAS11200 (discontinued) | 20 | 3.2 | 25 | 80 | 500 |
| CAS11201 (discontinued) | 20 | 3.2 | 50 | 160 | 1000 |
| CAS11729 (discontinued) | 40 | 6.4 | 12.5 | 80 | 500 |
| CAS11730 (discontinued) | 40 | 6.4 | 25 | 160 | 2 x 500 |
