PerkinElmer offers a trusted line of reagents, transfer membranes and secondary antibodies for highly sensitive and reliable detection quantitation of proteins and nucleic acids.
Blotting reagents | Transfer membranes | Secondary antibodies |
Western blot reagents
For the most sensitive detection and consistent performance, researchers can trust the Western Lightning™ brand of blotting reagents for chemiluminescent and chromogenic detection.
Western Lightning ECL Pro
Detect proteins in the low picogram range. The unique non-toxic formulation provides a long-lasting luminescent signal with an excellent dynamic range.
Western Lightning Ultra
Detect proteins in the low femtogram range . Ideal for conservation of precious samples.
Chromogenic substrates
- Use DAB to detect horseradish peroxidase (HRP)
- Use BCIP/NBT to detect alkaline phosphatase (AP)
Hybridization transfer membranes
Ensure accurate detection with high performance transfer membranes for blotting and other applications.
- Nitrocellulose membranes for high protein binding capacity and retention of small proteins in Western blotting
- Nylon membranes for nucleic acid binding in Northern and Southern blotting
- Charged nylon membranes for radiometric assays
Protran™
Protran 100% pure nitrocellulose membranes, with no cellulose acetate added, ensure the highest binding capacity possible.
GeneScreen™
Ideal for chemiluminescent and chromogenic techniques, as well as Northern and Southern blotting applications.
GeneScreen™ plus
Charged nylon hybridization transfer membranes are ideal for radiometric techniques.
Antibodies for chromogenic detection
anti-Digoxigenin | Goat anti-human IgG |
anti-Fluorescein | Goat anti-mouse IgG |
Streptavidin | Goat anti-rabbit IgG |
PhenoVue™ fluorescent secondary antibodies
- Highly cross-adsorbed antibodies minimize cross-reactivity
- Anti-rabbit or anti-mouse antibodies for common applications
- Validated in high-content screening applications