PhenoVue™ secondary antibodies
The range of PhenoVue™ fluor-labeled secondary antibodies offer bright signals for high quality images, and have been extensively validated in high-content screening applications. Highly cross-adsorbed anti-rabbit and anti-mouse antibodies minimize cross-reactivity in multiplexing experiments.
PhenoVue™ fluorescent secondary antibodies can be used in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
Goat anti-IgG (H+L) antibodies recognize IgG1, IgG2a, IgG2b and IgG3 isotypes. All antibodies are affinity purified.
Fluorescent label options include PhenoVue 488 (bright green), PhenoVue 568 (bright red-orange) and PhenoVue 594 (bright red).
- Cross-adsorbed antibodies may display cross-reactivity with other species. For improved specificity and lower background, or in multiplexed applications, consider using highly cross-adsorbed PhenoVue conjugated secondary antibodies as an alternative.
- Highly cross-adsorbed antibodies have been cross-adsorbed against various IgG species such as rat, hamster, human, rabbit, bovine, horse, or chicken serum to minimize cross-reactivity with other species.
| Vials per unit | 1 |
| Volume per vial | 1 mg |
| Form | Lyophilized |
| Storage | 2-8°C |
| Recommended working concentration | 1.5 µg/mL (10nM) |
| Max Ex/Em wavelengths (nm) |
495/520 (Fluor 488) |
| Common filter set | FITC (Fluor 488) Texas Red (Fluor 568, Fluor 594) |
| Cell staining | Fixed only |
| Equivalent number of microplates | 25-70 x 96-well microplates 20-70 x 384-well microplates 35-90 x 1536-well microplates |
Certificate of analysis
Safety data sheets
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