PhenoVue™ secondary antibodies
PhenoVue™ fluorescent secondary antibodies can be used in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
Goat anti-IgG (H+L) antibodies recognize IgG1, IgG2a, IgG2b and IgG3 isotypes. All antibodies are affinity purified.
Fluorescent label options include PhenoVue 488 (bright green), PhenoVue 568 (bright red-orange) and PhenoVue 594 (bright red).
- Cross-adsorbed antibodies may display cross-reactivity with other species. For improved specificity and lower background, or in multiplexed applications, consider using highly cross-adsorbed PhenoVue conjugated secondary antibodies as an alternative.
- Highly cross-adsorbed antibodies have been cross-adsorbed against various IgG species such as rat, hamster, human, rabbit, bovine, horse, or chicken serum to minimize cross-reactivity with other species.
|Vials per unit||1|
|Volume per vial||1 mg|
|Recommended working concentration||1.5 µg/mL (10nM)
|Max Ex/Em wavelengths (nm)||
495/520 (Fluor 488)
|Common filter set||FITC (Fluor 488)
Texas Red (Fluor 568, Fluor 594)
|Cell staining||Fixed only|
|Equivalent number of microplates||25-70 x 96-well microplates
20-70 x 384-well microplates
35-90 x 1536-well microplates
Certificate of analysis
Safety data sheets
PhenoVue diluent for fluorescent dyes dilution.
The PhenoPlates line of microplates have been engineered to deliver both performance and superior images for high-content application
The ViewPlate™ is specifically engineered for superior results in bottom reading systems.