Fluorescent & Hapten Nucleotides
Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
Fluorescent labels
| Label | Color | Laser (nm) | Filter (nm) | ex/em (nm) |
|---|---|---|---|---|
| Cyanine 3 (Cy3) | orange | 561 | 585/20 | 555/569 |
| Cyanine 5 (Cy5) | far-red | 640 | 670/30 | 651/670 |
| Diethylaminocoumarin | blue | 426/480 | ||
| Fluorescein | green | 488 | 525/50 | 494/517 |
| Lissamine™ | yellow | 561 | 570/588 | |
| Rhodamine 110 (R110) | green | 488 | 530/30 | 500/525 |
| Rhodamine 6G (R6G) | green | 532 | 575/25 | 525/560 |
| ROX | orange | 561 | 586/15 | 580/605 |
| TAMARA | yellow | 561 | 582/15 | 552/578 |
| Texas Red | orange | 561 | 582/15 | 586/603 |
Note: These analogs are intended to be detected directly by their fluorescence properties.
Cyanine smart pack
Each Cyanine Smart Pack contains 5 tubes each of cyanine 3 and cyanine 5 labeled nucleotide analog. Smart packs are ideal for microarray labeling and other molecular biology applications.
Hapten labels
| Label | Detection method | Linker arm length |
|---|---|---|
| Aminodigoxigenin (DIG) | anti-DIG-HRP conjugate | 9 |
| Biotin | streptavidin-HRP conjugate | 11 |
| Dinitrophenol (DNP) | anti-DNP-HRP conjugate | 11 |
Nucleotide |
Abbreviation |
Description/use |
|---|---|---|
| Adenosine triphosphate | ATP |
NEN brand fluorescent nucleotide analogs are biologically active with a variety of DNA and/or RNA polymerases, including Taq, Klenow fragment, SP6, T3, T7, and Sequenase™. Labeling methods such as nick translation, random priming, polymerase chain reaction, 3'-end labeling, or in vitro transcription may be used. Labeled probes may be used in applications including (but not limited to) microarrays and chromosome mapping. |
| Cytidine triphosphate | CTP | |
| Guanosine triphosphate | GTP | |
| Uridine triphosphate | UTP | |
| Deoxyadenosine triphosphate | dATP | |
| Deoxycytidine triphosphate | dCTP | |
| Deoxyguanosine triphosphate | dGTP | |
| Deoxyuridine triphosphate | dUTP | |
| Dideoxyadenosine triphosphate | ddATP | Biologically active and can be incorporated with a variety of DNA polymerases. Terminates further incorporation and enables identification of the complementary base for applications like DNA sequencing and detection of point mutations. |
| Dideoxycytidine triphosphate | ddCTP | |
| Dideoxyguanosine triphosphate | ddGTP | |
| Dideoxyuridine triphosphate | ddUTP |
Note: Some analogs demonstrate variations in relative performance depending upon nucleotide and fluorophore selection due to enzyme preferences.