Luminescence assays

Luminescence assays allow for the study of transcriptional gene expression, virus life cycles, and cell viability, making them significant tools for drug development in reporter gene, cytotoxicity, and cell proliferation applications, as well as general kinase activity monitoring.

PerkinElmer's luciferase-based reporter gene and ATP-monitoring assay systems, offer a wide dynamic range and greater sensitivity with lower interference than other detection options.

Reporter gene assay systems

The lites suite of luciferase detection reagents offer superior performance in an easy-to-use format, with the following benefits:

Convenient one-step processing: After cell lysis, simply dispense and read immediately
No DTT: Unlike most luciferase detection reagents, lites products do not contain DTT, eliminating toxic hazards and the need for hood work
Excellent Z' Values: All lites products offer robust, sensitive performance, with high signal-to-background ratios and excellent Z' values

britelite™ plus

Our brightest luciferase reporter gene assay

An ultra-high sensitivity system for the quantitation of firefly luciferase expression in mammalian cells. Ideal for smaller-scale sample analysis when maximum sensitivity is needed

steadylite™ plus

Our longest-lasting signal for luciferase quantitation Provides an extended signal half-life of more than 4 hours. Ideal for high-throughput assays employing batch processing methodology.

neolite™

Bright signal and long-lasting signal

Signal half-life of more than 2 hours at high signal intensity. Designed for systems that require increased sensitivity with the benefit of an extended detection time.

sensilite™

For highly sensitive and rapid luciferase detection

A dispense-and-read, ultra-high sensitivity system for high-throughput assays with continuous processing methodology. For use with luminometers equipped with dispensers.

twinlite™

For dual luciferase detection in cultured cells

A dual luciferase reporter gene assay system for the quantitation of Firefly and Renilla luciferase expression in mammalian cells. For use with luminometers equipped with two dispensers.

ATP assays

ATP is a useful marker for cell viability due to its presence in all metabolically active cells. ATP concentration declines rapidly when cells undergo necrosis or apoptosis, and so ATP monitoring can be used to assess the cytocidal, cytostatic and proliferative effects of a wide range of drugs, biological response modifiers and biological compounds.

Our ATPlite and ATPlite 1step luminescence assay systems use patented innovative technologies that measure cell proliferation and cytotoxicity in cultured mammalian cells or 3D spheroids based on the production of light caused by the reaction of ATP with added luciferase and D-luciferin.

ATPlite™ luminescence assay system

For quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells

Long-lived luminescent signal - half-life greater than 5 hours
Rapid results - generated in 15 - 25 minutes
Sensitive down to 5 cells in 100µL medium

ATPlite™ 1step luminescence assay system

Simple and reproducible - only one reagent addition step, no separation steps

Designed for continuous process systems
High sensitivity down to one cell per well
Available for cultured cells or 3D spheroids

Western blotting reagents now available