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neolite™ reporter gene assay system

The neolite™ system offers the ultimate compromise between strong signal intensity and extended signal half-life. It provides a minimum half-life of 2.5 hours while maintaining excellent sensitivity. neolite also delivers superior reproducibility, as it has less sensitive mixing conditions than typical luminescent detection systems. It is ideal for medium to high throughput applications which employ batch processing methods but still require exceptional sensitivity.

The neolite™ system is a homogeneous, high sensitivity, long-lived glowtype firefly luciferase reporter gene assay for the quantification of firefly luciferase expression in mammalian cells. The reagent formulation contains compounds that facilitate both cell lysis as well as the luciferase enzymatic reaction. neolite has been designed to cover the applications where a high light intensity is required together with an extended half-life. neolite is designed for systems that require increased sensitivity with the benefit of an extended detection time. neolite generates a luminescent signal that is both bright and stable. In general neolite has a signal half-life of more than 2 hours at high signal intensity.

As with all members of the 'lites' suite of luciferase detection reagents, britelite plus offers superior performance in an easy-to-use format, providing the following benefits:

  • Convenient one-step processing: No washes required - simply mix, incubate and read
  • Enhanced stability: All lites reagents are designed for extended storage at 2 - 8 °C
  • No DTT: Unlike most luciferase detection reagents, lites products do not contain DTT, eliminating toxic hazards and the need for hood work
  • Excellent Z' values: All lites products offer robust, sensitive performance, with high signal-to-background ratios and excellent Z'values