Estimate the quantities of siRNA/microRNA and DharmaFECT for your high-throughput screening experiments.
Remember to include consideration for:
- Investigations in multiple cell lines
- Pipetting error allowances
- Reserves for secondary screening
| Typically 20-50 nM. Accell siRNA is 1000 nM (1 µM). | ||
| 100 μL is typical working volume in 96 well plate. | ||
| E.g. 30 screens in triplicate, enter 90. | ||