Ubiquitination pathway

The ubiquitination pathway has been implicated in a number of genetic disorders and diseases including cancer and neurological diseases which result from the build-up of protein aggregates such as; Alzheimer’s disease, Parkinson’s disease and Huntingdon’s disease. This collection has been generated in close collaboration with Prof. Steve Jackson (Gurdon Institute, Cambridge, United Kingdom).

Deubiquitination image

Experimental validation

Deubiquitination of FANCD2 by USP1 is impacted in a USP1 knockout cell lines.

Cells were washed with PBS, lysed in Frackelton buffer (10 mM Tris/HCl pH 7.5, 50 mM NaCl, 30 mM sodium pyrophosphate, 1 % Triton X-100, 50 mM NaF and protease inhibitors).

They were analyzed by SDS-PAGE and Western blotting using anti-FANCD2 (sc-20022 from Santa Cruz). HAP1 wild-type cells were compared to cells bearing a frameshift mutation in USP1.

 
 
 
 
Explore our popular knockout cell lines for deubiquitinating enzymes genes
ATXN3 COPS6 USP22 UCHL3 USP10 USP14
OTUD5 PAN2USP47 USP27X USP30 VCPIP1
USP18 USP20 CYLD USP53 USP7 OTUB1
USP4 USP45 TDG UNG OTUD3 USP11
BRCC3 COPS7AUSP24 USP28 USP15 USP31
OTUD6B STAMBP USP48 USP54 ZRANB1 OTUB2
USP19 USP21 UCHL1 MPND OTUD4 USP13
USP40 USP46 USP25 USP1 USP16 USP32
OTUD7B COPS7B USP49USP6
USP38
USP2 STAMBPL1 JOSD1 MYSM1 OTUD1