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Guide RNA Designer

For use with S.pyogenes Cas9 (synthetic sgRNA, crRNA, or lentiviral sgRNA)

Enter a 17-23 nt target sequence. Repeat region will be appended automatically

For use with any Cas9 species

crRNA: Enter 35-45 nt consisting of target sequence and repeat region

Synthetic sgRNA: Enter 97-103 nt consisting of target sequence, repeat region, linker, and tracrRNA

Pin-point™ system: For use with Rat APOBEC and nCas9 mRNA (synthetic sgRNA)

Enter a 20 nt target sequence. Repeat region will be appended automatically

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