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Pro(tein) Validated Engineered Cell Lines
Functional testing, powered by Revvity Reagents.
Our Pro(tein) Validated Engineered Cell Lines are expertly verified with targeted antibodies to give you the confidence you need that our knock-out cell lines have both a loss of gene and protein expression, giving power to your functional studies.
Why choose our pro(tein) validated cell lines?
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Verified for both loss of gene and loss of protein expression
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Purpose-built for functional studies
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HTRF™ assay-backed verification with our homogeneous time-resolved fluorescence technology
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Validated with BioLegend™ antibodies for comprehensive protein-level confirmation
Different Forms of Validation
Revvity has an extensive collection of HTRF™ (Homogeneous Time Resolved Technology) assays, a FRET-based, no-wash technology with a wide range of applications. HTRF assays provide key data for knockout cell line validation. A loss of fluorescence signal when comparing wild-type cells with engineered cells provides high levels of confidence that our knockout cell lines lack functional protein of the targeted gene.
BioLegend™ antibodies are designed to target unique epitopes on specific proteins, and they can be optimized for a variety of readouts, including western blot, flow cytometry, immunofluorescence, immunocytochemistry, and immunohistochemistry. Using these antibodies across different applications can validate our cell lines. A loss of signal when comparing wild-type to knockout cells demonstrates a loss of protein expression of target gene.
Illustration of HTRF assay. Two antibodies, one with a donor and one with an acceptor fluorophore, bind to distinct epitopes on target proteins.
Learn more about HTRF assays
Learn more about BioLegend antibodies
Explore our protein-validated collection
New cell lines added regularly.
| ATG16L1 | BRD9 | CDK5 | NRF2 | ATXN2 | RAF1 |
| BRD2 | STAT6 | CDK8 | CHK2 | HSP27 | NPM1 |
| BRD3 | H2AFX | XBP1 | MYD88 | CCNE1 | SRC |
| BRD7 | SMARCA-4 | IKKa | KRAS | ATF4 | LDHA |
| TJP1 | MSI2 | KDM3B | UCHL1 | SMAD4 | CAS9 |
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