This tool is for designing and ordering a single-strand DNA donor (≤ 150 nt) for precise CRISPR-Cas9 gene editing with the HDR pathway. The donor-oligo is modified to provide a more than 2-fold improvement in knock-in efficiency (vs non-modified oligo).
To design a crRNA to cut near the genomic alteration site of interest, first use the CRISPR Design Tool.
If you already have a guide RNA that is near the desired alteration site, enter the gene information and 20 nt guide RNA target sequence below.
Need some additional guidance? Visit the HDR Donor Designer Workflow page to learn more.