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By assessing phenotypes for thousands of designs, we established standards for identifying target sites that are more likely to achieve functional protein knockout while retaining high levels of specificity
Supports 30+ species, alternative nucleases beyond standard SpCas9, and can design guides not just in the protein-coding region but also for microRNAs, long non-coding RNAs, and intronic regions
Custom guide designs can be made as a synthetic guide or packaged into any of our lentiviral vectors, including the All-in-one vector, which combines SpCas9 sequence and a gene-specific guide RNA for a single, ready to use reagent.