Estimate the quantities of siRNA/microRNA and DharmaFECT for your high-throughput screening experiments.

Remember to include consideration for:

  • Investigations in multiple cell lines
  • Pipetting error allowances
  • Reserves for secondary screening
Typically 20-50 nM. Accell siRNA is 1000 nM (1 µM).
100 μL is typical working volume in 96 well plate.
E.g. 30 screens in triplicate, enter 90.