Learn how antisense oligonucleotides (ASO) are maximizing treatment efficiency and reducing off-target effects in combinatorial therapies.
In this blog post, we share the highlights of our CRISPRmod CRISPR interference (CRISPRi) system, comparing the activity of Dharmacon’s novel CRISPRi construct dCas9-SALL1-SDS3 to dCas9-KRAB.
CRISPRによる全ゲノムスクリーニングを開始しましょう。新しいDharmacon all-in-one CRISPRプラットフォームなら、これまで以上に簡単に実行できます。
Design and order a plasmid DNA donor kit for insertion of a fluorescent marker
Arrayed siRNA screens are a powerful tool to look at very precise perturbations in cellular pathways.
HAP1細胞の概要、その起源、そしてこれらの細胞株でノックアウトを生成する方法について、お客様の研究にどのように役立つかをご紹介します。
T-cell receptors (TCRs) are crucial components of T cell immunity, enabling the detection and subsequent elimination of abnormal, cancerous and infected cells. Dysfunctional T cell receptor signaling can lead to various immune deficiency or autoimmune diseases. In cancer treatment the study and modification of T cell receptor signaling is important for CAR-T cell therapy as well as modulation of the tumor microenvironment (TME). Modulating T cell activation can be important to avoid rejection of organ or stem cell transplants by the immune response.
Cancer is often characterized by dysregulation in cellular pathways and the checkpoints which control cell proliferation, growth, differentiation, and apoptosis. Within the genome there are two types of genes which carefully control cell proliferation and cell death, which are often the driving force behind oncogenesis.
Revvity’s Dharmacon™ CRISPR portfolio expands with validated CRISPR guide RNAs for complex but critical gene targets, as well as DNA cutting controls for screen normalization in sensitive assays.
3D culturing is a valuable and increasingly popular cell culture technique. This model recapitulates some physiological characteristics of tissues and tumors driven by cell-cell and cell-matrix contacts, circumventing the need for in vivo experiments in some cases.