ON-TARGETplus™ 2.0 siRNAおよびAccell™ 2.0 siRNAスクリーニングライブラリーについては、こちらをクリックしてください。
Dharmacon™ ON-TARGETplus™ 2.0 および Accell 2.0 siRNA を使用することで、お客様の遺伝子サイレンシング試薬は最新のトランスクリプトームデータと常に整合した状態を維持できます。継続的なリアラインメント手法により、最新の注釈を反映したsiRNA設計が可能となり、意図しないオフターゲット効果のリスクを低減しつつ、一貫した遺伝子ノックダウンを実現するように選定されています。
独自のsiRNA SMARTpoolおよび4種類のIndividual siRNAセットのフォーマットと組み合わせることで、最新化されたsiRNAライブラリーコレクションにより、現代の創薬ワークフローにおいて、より高い信頼性でsiRNAによる遺伝子サイレンシングを実施できます。
Accell 2.0ライブラリーは、最適化された自己送達型化学修飾により、初代細胞やトランスフェクションが困難な細胞モデルにおいても、トランスフェクション試薬を用いることなく遺伝子サイレンシングを実現します。
組み合わせ済みのライブラリーに含まれない独自の遺伝子リストを使用したい場合は、Cherry-pick カスタムアレイプレーティングツールをご利用いただくことで、お客様独自のスクリーニングライブラリーを作製できます。
提供している各種siRNAスクリーニングライブラリーについては、以下の一覧をご覧ください。
ON-TARGETplus 2.0 siRNA Screening Libraries
Accell 2.0 siRNA Screening Libraries
|
ON-TARGETplus 2.0 |
Accell 2.0 |
| GPCRs |
✔ |
✔ |
| Ion Channels |
✔ |
✔ |
| Phosphatases |
✔ |
✔
|
| Proteases |
✔ |
✔
|
| Protein Kinases |
✔ |
✔ |
| Ubiquitin Enzymes |
✔ |
✔ |
| Transcription Factors |
✔ |
✔ |
| Drug Targets |
✔ |
✔ |
| Ubiquitin Conjugation Subset 1 |
✔ |
✔ |
Ubiquitin Conjugation Subset 2
|
✔ |
✔ |
| Ubiquitin Conjugation Subset 3 |
✔ |
✔ |
| Apoptosis |
✔ |
✔ |
| Cell Cycle Regulation |
✔ |
✔ |
| Cytokine Receptors |
✔ |
✔ |
| Deubiquitinating Enzymes |
✔ |
✔ |
| DNA Damage Response |
✔ |
✔ |
| Epigenetics |
✔ |
✔ |
Membrane Trafficking
|
✔ |
✔ |
| Nuclear Receptors |
✔ |
✔ |
| Tyrosine Kinases |
✔ |
✔ |
Which siRNA is right for you?
Dharmacon reagents offers multiple predesigned product lines across human, mouse and rat genomes. Use the table below to assist you in determining the right siRNA product line for your needs.
| Feature / Attribute |
ON-TARGETplus 2.0 siRNA |
Accell 2.0 siRNA |
ON-TARGETplus siRNA |
siGENOME siRNA |
Lincode siRNA |
| Key Benefit |
Next-generation specificity with minimized off-target effects and improved consistency |
Next-generation specificity to target silencing in difficult-to-transfect cells |
High specificity for reduced off-targets + efficient silencing |
Cost-effective, efficient silencing |
Highly specific knockdown of long noncoding RNA (lncRNA) |
| Gene Coverage |
Predesigned for Human, Mouse, and Rat protein-coding genes |
Predesigned for Human, Mouse, and Rat protein-coding genes |
Predesigned for Human, Mouse, and Rat protein-coding genes |
Predesigned for Human, Mouse, and Rat protein-coding genes |
Predesigned for Human and Mouse lncRNA |
| Best Use Case |
Recommended for high-specificity screening, functional genomics, and follow-up validation |
Recommended for neuronal, suspension, primary, and other hard-to-transfect cells |
Recommended for transfectable mammalian cells |
Recommended for transfectable mammalian cells |
Highly specific lncRNA targeting |
| Transfection Requirements |
DharmaFECT reagent recommended |
None required |
DharmaFECT reagent recommended |
DharmaFECT reagent recommended |
DharmaFECT reagent recommended |
| Available as SMARTpool format |
✔ |
✔ |
✔ |
✔ |
✔ |
| Available as 4 individual siRNAs |
✔ |
✔ |
✔ |
✔ |
✔ |
| Guaranteed Knockdown (SMARTpool format + 3 of 4) |
✔ (updated design enhances reliability) |
✔ |
✔ |
✔ |
— |
| Sense Strand Modifications |
✔ |
✔ |
✔ |
Selective application as needed |
✔ |
| Antisense Seed Region Modification |
✔ |
— |
✔ |
— |
✔ |
| Uptake-Enhancing Modifications |
— |
Modifications to support delivery |
— |
— |
— |
| Nuclease Resistance |
— |
Stabilizing modifications included |
— |
— |
— |
| Sequence Information Provided With Purchase |
✔ |
✔ |
✔ |
✔ |
✔ |
| Product Search |
Search ON-TARGETplus 2.0 |
Search Accell 2.0
|
Search ON-TARGETplus siRNA |
Search siGENOME |
Search Lincode |