Cas9発現細胞株

Cas9を安定的に発現させた細胞株で遺伝子編集実験を簡素化

汎用される細胞株バックグラウンドから選択できます。CRISPRガイドRNAをトランスフェクトするだけで、CRISPRノックアウト実験を開始できます。

ゲノム編集実験およびloss-of -function実験のために：

スクリーニングプロジェクトのためには：

Horizon Discoveryの細胞株をご購入の際は、細胞株の限定使用ラベルライセンス（LULL: LIMITED USE LABEL LICENSE AGREEMENT）へのご同意が必要です。

Cas9発現細胞株用細胞培養培地一覧

Cell line background	Cat #	Basal medium	Supplements	Subculturing tips
A549	HD Cas9-001	RPMI 1640	10% FBS 1% Pen/Strep	Recommended split ratio - 1:3 to 1:8 Maintain cultures between 6 x 103
HCT-116	HD Cas9-002 HD dCas9-VPR-002	RPMI1640	10% FBS 1% Pen/Strep	Recommended split ratio - 1:3 to 1:8 Split cultures at 75-90% confluency
Jurkat	HD Cas9-004	RPMI1640	10% FBS 1% Pen/Strep	Maintain cultures between 1 x 105and 1 x 106cells/mL
K-562	HD Cas9-005 HD dCas9-VPR-005	IMDM	10% FBS 1% Pen/Strep	Maintain cultures between 1 x 105and 1 x 106cells/mL
RKO	HD Cas9-007	RPMI1640	10% FBS 1% Pen/Strep	Recommended split ratio - 1:3 to 1:12 Split cultures at 75-90% confluency
NIH/3T3	HD Cas9-010	DMEM, high glucose, GlutaMAXTM	10% FBS 1% Pen/Strep	Seed 3-5 x 103cells/cm2 Split cultures at 80% confluence or less Do not allow the cultures to become confluent
HAP1	HD Cas9-011 HD dCas9-VPR-011	IMDM	10% FBS 1% Pen/Strep	Recommended split ratio - 1:10 - 1:15 Do not allow cells to be kept at high density, maximum density: 75% confluency
U2OS	HD Cas9-012 HD dCas9-VPR-012	DMEM, high glucose	10% FBS 1% Sodium Pyruvate 1% Pen/Strep	Recommended split ratio - 1:3 to 1:6 Split at 75-90% confluency