ゲノム編集とは、細胞DNAの領域を変更するプロセスを指します。最も一般的なゲノム編集技術には、遺伝子の機能の不活性化(ノックアウト)、SNP変異の導入または修正、または内因性遺伝子へのレポータータグの追加(ノックイン)が含まれます。これらの変化は永続的で遺伝性であり、新たに改変細胞株が得られます。
Edit-R™ CRISPR-Cas9試薬を使用すると、研究者は、実質的にあらゆる細胞タイプのあらゆる遺伝子に対して、標的した箇所に特有の編集を行うことができます。さらに、Horizonは、幅広い遺伝子改変済み細胞株とゲノム編集関連研究サービスも提供しています。

Precision LentiORFs

Precision LentiORFs are expression-ready open reading frames (ORFs) that have been cloned into a lentiviral expression vector with a GFP reporter.

CCSB Human ORFeome Collection

Gateway® adapted human entry ORFs are ideal for easily moving entire families of ORFs into your choice of protein expression systems.

Horizon-One-Web-Screening-Category-Page-Hero-2880x500
Cherry-pick library tool

Design your own custom cDNA or ORF libraries

CCSB-Broad Lentiviral Expression Collection

Human ORFS in a lentiviral vector, stop codons have been removed and clones contain a C-terminal V5 tag.

ORFeome Collaboration Clones

ORFeome Collaboration clones are sequence-verified, human open reading frames (ORFs) subcloned into Gateway® recombinational entry vectors.

hORFeome v8.1 Collection

This fully-sequenced collection of Gateway-adapted ORFs represents the latest version of the CCSB Human ORFeome library from Dana-Farber Cancer Institute.

Non-mamalian cDNAs and ORFs

Overexpress genes from Yeast, C. elegans, E. coli, Zebrafish or Xenopus laevis.

Precision lentiORF

RNAi Rescue using Precision LentiORF

The Precision LentiORF collection provides a comprehensive set of ready-to-use clones that permit RNAi rescue in studies that utilize 3'-UTR siRNA reagents. This study highlights the necessary controls and optimization required for an RNAi rescue experiment and demonstrates the utility of these constructs when validating phenotypes associated with siRNA-mediated silencing.

Top five considerations for ORF selection

Five tips to quickly and effortlessly select the right clone format for your over-expression project.

Using ORFs with variable stop positions

Many Precision LentiORF clones are available either with or without the native stop codon.