Next generation sequencing (NGS) platforms give researchers much greater power when profiling tumor samples. However, the use of NGS presents challenges surrounding sample variability, platform bias, and potential failure to detect biomarkers. Horizon’s standards are appropriate for any NGS library preparation including whole-genome, whole-exome, custom capture and targeted amplicon panels. Horizon has developed this Quality-Seq (Q-Seq) NGS Reference Standard range to support the development and continued validation of Next Generation Sequencing platforms. The Gene-Specific Multiplex Reference Standard portfolio covers multiple endogenous SNPs, insertions and deletions at defined allelic frequency ranges. The Gene-Specific Reference Standards cover EGFR or KRAS-specific mutations. These standards may be diluted with a matched wild type while the background mutations remain consistent. The Gene Specific Multiplex Standards are available in genomic DNA and FFPE format, which allows you to validate both the pre-analytical and analytical portion of your workflow. The FFPE format allows you to evaluate and optimize DNA extraction protocols without wasting precious patient samples. By multiplexing multiple gene-specific mutations in a single standard, the Gene-Specific Multiplex allows researchers to maximize the information obtained from their assay, and confirm that they are able to detect a range of potential mutants in a single gene.

With this product you are able to:

• Evaluate of workflow integrity from pre-analytical DNA extraction to post-analytical bioinformatics
• Analyze the sensitivity of your workflow
• Optimize and validate new cancer panels and routinely monitor the performance of your assay

Product Information

Product Description: EGFR Gene Specific Multiplex Reference Standard

Catalog Number: HD300

Format: FFPE

Genes Covered: EGFR

Fixation Method: 4%PFA (w/v)

Section Size: 15 µm

Cell Density: 3 x 10⁸ cells per block. Approx. 3.5 x 10⁵ cells per section

Expected DNA Yield: ≥ 400 ng using Promega Maxwell RSC Plus Extraction kit

Verified Mutations:

Presence confirmed in parental cell line:

^* GA>G at hg19|chr16:68867504 and hg38|chr16: 68833601 (rs553402231)

Unit Size: 1 FFPE Section

Extractable DNA: ≥ 400 ng per section using Promega Maxwell RSC Plus FFPE DNA Extraction kit

Exome sequencing data: Download additional information on all exonic variants present in this product. Please note: this NGS data is not batch specific.

General Information

Allelic Frequency: 5%

Storage: 4˚C

Shipping: Ambient

Expiry: See all product shelf life information

Quality Control

Genotype and Allelic Frequency: Droplet Digital PCRTM

DNA Integrity: Agarose gel electrophoresis

Quantification: Quantifluor™

Manufacturing Quality: ISO 13485:2016

Intended use: For assay developers and molecular diagnostic labs for routine performance monitoring of molecular biology assays. Research use only. Not for diagnostic procedures.