Mimix™ Quantitative Multiplex, FFPE Reference Standard IVD
Mimix™ Quantitative Multiplex FFPE Reference Standard is a highly characterized, biologically-relevant quality control material used to assess the performance of NGS and PCR assays that detect somatic mutations.
For In Vitro Diagnostic use. This product is only available where licensed in accordance with law. Please contact your local representative for availability.
Mimix quantitative multiplex control is a cell line-derived pan-cancer reference standard, featuring 11 variants across 6 key cancer genes. These variants range from 0.9-24.5% allelic frequency (AF), to aid in the limit of detection (LOD) determination of your assay. Each batch of Mimix quantitative multiplex controls include 11 variants confirmed by ddPCR, along with a batch-specific whole exome sequencing (WES) variant allelic frequency (VAF) file at 500x coverage performed by Revvity Omics using the Agilent CREV3 kit on an Illumina or Aviti platform. This provides an accurate and reliable truth set for monitoring your workflow and assay's performance.
Technical Product Information
Format: FFPE
Fixation method: 4% PFA
Section size: 15µm
Cell density: 3 x 108 cells per block. Approx. 3.5 x 105 cells per section
Expected DNA yield: > 400 ng
Genes covered: BRAF, KIT, EGFR, KRAS, NRAS, PIK3CA
Allelic frequencies: 0.9-24.5%
| Chromosome | Gene | Variant | Expected Allelic Frequency, % |
|---|---|---|---|
|
7q34 |
BRAF |
V600E |
10.70% |
|
4q11-q12 |
cKIT |
D816V |
10.00% |
|
7p12 |
EGFR |
ΔE746 - A750 |
1.90% |
|
7p12 |
EGFR |
L858R |
2.80% |
|
7p12 |
EGFR |
T790M |
0.90% |
|
7p12 |
EGFR |
G719S |
24.50% |
|
12p12.1 |
KRAS |
G13D |
15.00% |
|
12p12.1 |
KRAS |
G12D |
6.30% |
|
1p13.2 |
NRAS |
Q61K |
12.50% |
|
3q26.3 |
PIK3CA |
H1047R |
17.50% |
|
3q26.3 |
PIK3CA |
E545K |
8.80% |
General information
Storage: 4°C
Shipping: Ambient
Expiration: See all product shelf life information
Intended Use:
The Mimix™ quantitative multiplex FFPE reference standard is a commutable control material comprising a formalin-fixed paraffin-embedded (FFPE) curl derived from well-characterized human cancer cell lines, containing 11 variants across 6 key cancer genes. It is intended for monitoring next-generation sequencing (NGS) or droplet digital polymerase chain reaction (ddPCR) assays designed to detect somatic mutations in genomic DNA (gDNA) from human samples for in vitro diagnostic use.
The gDNA obtained from the Mimix quantitative multiplex FFPE reference standard can be used to monitor NGS or ddPCR workflow, test performance, assay variation, and helps identify increases in random or systematic errors. This product is for professional laboratory use only.
Quality control
Allelic frequency: Droplet Digital™ PCR
Quantification: Quantifluor™
DNA Integrity: Agarose gel electrophoresis
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To order offline we will require the following information:
- Product code(s) of the item(s) you wish to order (e.g. "HD123")
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