Lincode GAS5 Control Pool is an effective positive control for RNAi experiments for knockdown of human long, noncoding RNAs (lncRNAs). Growth arrest-specific 5 (GAS5) is expressed during growth arrest phase of the cell cycle. The four component Lincode GAS5 siRNAs are designed to target various regions of the GAS5 transcript, providing more efficient overall knockdown results. Each siRNA carries a proprietary dual-strand modification patterns to have fewer off-targets than unmodified siRNAs. Endogenous GAS5 expression levels should be determined prior to experiments with this reagent.

Highlights

• Positive control for an endogenous lncRNA in human cells
• Chemically matched for use with Lincode siRNA reagents
• Effective silencing verified in multiple cell types

Application considerations

Prior to beginning lncRNA knockdown experiments, the expression level of GAS5, or other target lncRNA, should be assessed in your cell line. If GAS5 levels are not reliably detectable, it is recommended to use ON-TARGETplus GAPD siRNA Control Pool or ON-TARGETplus Cyclophilin B Control Pool as a means of assessing transfection and RNAi efficiency.