The miRIDIAN microRNA Hairpin Inhibitor Positive Control is ideal for optimizing conditions for relevant, well-controlled microRNA modulation experiments. Both positive and negative control molecules are provided for loss-of-function microRNA studies.

Successful microRNA functional studies begin with optimization of the assay in each cell line and/or type of interest. To optimize inhibition experimental conditions by assessing the effect of inhibition on an endogenously expressed microRNA, we offer a validated Hairpin Inhibitor Positive Control.

Highlights

• Targets endogenous miR-16 in human, mouse, and rat cells resulting in reduced miR-16 levels (seeFigure 1, Supporting Data)
• Control designs and modifications are identical to experimental miRIDIAN microRNA Hairpin Inhibitors

Applications

• Optimize microRNA inhibition assays by targeting a microRNA in a cell line that expresses miR-16
• Specific inhibition of miR-16 can be directly assessed by RT-qPCR and/or Northern blotting