miRIDIAN microRNA Mimic Endogenous Positive Control
A validated miRIDIAN microRNA Mimic designed to target Aldolase A in human, mouse, and rat
The miRIDIAN microRNA Mimic Endogenous Positive Control helps you monitor specific mimic effects on target protein levels in a validated endogenous assay, which is based upon the targeted activity of miR-122 on Aldolase A mRNA levels in cell lines.
miRIDIAN microRNA Mimic Positive Controls are ideal for optimizing conditions for relevant, well-controlled microRNA modulation experiments. Both positive and negative control molecules are provided for overexpression microRNA studies.
Successful microRNA functional studies begin with optimization of the assay in each cell line and/or type of interest. To optimize experimental conditions, we offer both housekeeping and endogenous positive controls for overexpression experiments using miRIDIAN Mimics.
Highlights
- Validated microRNA Mimic that targets Aldolase A in human, mouse, and rat
- Same structure and design as experimental miRIDIAN microRNA Mimics
- Allows optimization of assay conditions by monitoring mimic function on an endogenous gene target (Aldolase A) with a conserved miR-122 binding site (see Figure 1, Supporting Data)
- Based upon the targeted activity of miR-122 on Aldolase A mRNA levels in cell lines, expressing low to moderate levels of miR-122
Applications
- Link the activity of a microRNA mimic to post-transcriptional regulation of its putative gene target
- Measure modulation of endogenous Aldolase A mRNA levels
