Edit-R CRISPR-Cas9 Gene Engineering Platform

The Dharmacon Edit-R Gene Engineering platform is powered by a proprietary algorithm and based on the Type II CRISPR-Cas9 system from the bacteria Streptococcus pyogenes.

• Ready-to-Use Components: The system eliminates the need for cloning, allowing researchers to quickly initiate experiments with predesigned guide RNAs and other essential components, significantly reducing the time to results.
• High Specificity and Efficiency: Edit-R utilizes a proprietary algorithm to design guide RNAs that improve the likelihood of achieving functional knockouts while minimizing off-target effects, ensuring high confidence in experimental outcomes.
• Guaranteed Performance: Every predesigned guide RNA is backed by a guarantee of successful editing at the target site, providing researchers with additional assurance in their gene editing endeavors.

The Edit-R predesigned guide RNA guarantee

We guarantee that EVERY predesigned guide RNA will provide successful editing at the target site when delivered as described in the Edit-R Technical Manuals.

The Edit-R guide RNA guarantee is valid when used with any wild type S. pyogenes Cas9 nuclease, including mRNA, expression plasmid, protein, or stable Cas9 expression, and Edit-R crRNAs must be used with Edit-R tracrRNA for the guarantee to apply.

Analysis of editing of the treated cell population must be shown using a T7EI or Surveyor mismatch detection assay. If successful editing is not observed for a predesigned Edit-R guide RNA while an appropriate side-by-side Edit-R positive control is successful, a one-time replacement of a different predesigned Edit-R guide RNA of the same format and quantity will be provided at no cost.

A replacement will only be approved upon discussion with our Scientific Support team.

Successful editing at the DNA level does not always lead to functional gene knockout; it is recommended to test multiple guide RNAs to determine the most effective guide RNA for knockout of your target gene.

This guarantee does not extend to any accompanying experimental costs, does not apply to guide RNAs ordered via the CRISPR Design Tool, and will not be extended to the replacement guide RNA.

Gene knockout workflow using the Edit-R All-in-one lentiviral sgRNA system

Gene knockout workflow with the All-in-one lentiviral sgRNA using a mixed popultion (left side) or clonal cell line (right side) experimental approach.

Edit-R lentiviral Cas9ヌクレアーゼおよびsgRNAベクターのベクターマップとベクターエレメント

Edit-R lentiviral Cas9 NucleaseおよびsgRNAベクターのベクターマップとベクターエレメント。Edit-R lentiviral CRISPR-Cas9プラットフォームは、Cas9発現を最大化するための複数のPol IIプロモーターオプションを持つレンチウイルスベクターと、目的の標的部位に設計されたsgRNA発現用の遺伝子特異的ベクターを利用する2ベクターシステムです。

Edit-R lentiviral sgRNAベクターのプラスミドベクターマップ

Edit-R lentiviral sgRNAベクターバックボーンでは、遺伝子特異的なcrRNAとtracrRNAがヒトU6プロモーターの制御下で発現します。一方、ピューロマイシン耐性マーカー（Puro^R）の発現はマウスCMVプロモーターから駆動され、sgRNAが組み込まれた細胞の迅速な選択を可能にします。このプラスミドには、大腸菌での増殖と選択のためのAmp^R耐性マーカーが含まれています。

Edit-R lentiviral sgRNAを用いた実験ワークフロー

Edit-R lentiviral sgRNAを用いた実験ワークフロー。Edit-R lentiviral sgRNAをCas9ヌクレアーゼを発現する安定細胞株に導入することで、低MOIでも効率的に遺伝子をノックアウトすることができます。