Pan-Cancer 6-Fusion Panel (Negative Control)
Horizon’s Pan-Cancer 6-Fusion Panel (Negative Control) FFPE RNA Reference Standard is a highly-characterized, biologically-relevant quality control material used to assess the performance of targeted NGS RNA-seq, endpoint reverse-transcription PCR (RT-PCR), RT-qPCR and RT droplet digital PCR (RT-ddPCR) assays aimed at detecting gene fusions.
This control was specifically designed to compliment Horizon's Pan-Cancer 6-Fusion Panel (HD834).
Each FFPE section is composed of a formalin-fixed, paraffin-embedded (FFPE) cell-line verified not to contain TPM3-NTRK1, QKI-NTRK2, ETV6-NTRK3, EML4-ALK, CCDC6-RET and SLC34A2-ROS1 fusions.
Technical Data
Format: FFPE
Genes Covered: Confirmed negative for TPM3-NTRK1, QKI-NTRK2, ETV6-NTRK3, EML4-ALK, CCDC6-RET, SLC34A2-ROS1
Fixation method: 10% Formalin
Section Size: 15 µm
Unit size: 1 x 15 µm FFPE section
Storage: 4˚C
Expiry: See all product shelf life information
Product Information
Verified Mutations:
| 5' Partner | 5' Partner Chromosome | 3' Partner | 3' Partner Chromosome | RNA Expression Level |
|---|---|---|---|---|
|
TPM3 (Exon 7) |
1 |
NTRK1 (Exon 10) |
1 |
≤2 Copies/ng |
|
QKI (Exon 6) |
6 |
NTRK2 (Exon 14) |
9 |
≤2 Copies/ng |
|
ETV6 (Exon 5) |
12 |
NTRK3 (Exon 15) |
15 |
≤2 Copies/ng |
|
EML4 (Exon 13) |
2 |
ALK (Exon 20) |
2 |
≤2 Copies/ng |
|
CCDC6 (Exon 1) |
10 |
RET (Exon 12) |
10 |
≤2 Copies/ng |
| SLC34A2 (Exon 4) |
4 |
ROS1 (Exon 32) |
6 |
≤2 Copies/ng |
Intended Use: For assay validation and routine monitoring of assay performance (for research use only)
Quality Control
Extractable RNA / Expected Yeild: ≥ 100 ng RNA per section using the Maxwell RSC® RNA FFPE Purification Kit (Promega)
RNA Quantification: Qubit® RNA HS Assay (Thermo Fisher Scientific)
Fusion Biomarker Expression: Absence of TPM3-NTRK1, QKI-NTRK2, ETV6-NTRK3, EML4-ALK, CCDC6-RET and SLC34A2-ROS1 RNA transcripts confirmed by fusion specific RT-ddPCR using the QX200 ddPCR System (Bio-Rad)
RNA Integrity: Expected DV200 ≥65% assessed using TapeStation High Sensitivity RNA ScreenTape Assay (Agilent)
We accept orders using the following methods.
Online
Our online catalog and ordering system can be used to search our full menu of available Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.
Offline
If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order. We recommend that you use our online system to identify the products you require and their respective “HD” product codes.
To order offline we will require the following information:
- Product code(s) of the item(s) you wish to order (e.g. "HD123")
- Your name, email address, title and organization
- Shipping address and billing address with contact names
- Purchase Order number if you have one
- Telephone number
- VAT Number (European Countries only)
If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please contact us.
Application notes
Certificate of analysis
Selection guides
Related Products
Defined FFPE control material containing 6 clinically-relevant, cell-based kinase fusion RNA biomarkers