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Dharmacon reagents | Infinite possibilities for genomic perturbation.
CRISPR, RNAi & Custom oligo synthesis. Genome-scale tools for gene knockout, knock-in, knockdown, activation, and more. 30+ years of proven expertise.
Mimix reference standards
Cell line-derived reference materials. Mimic patient samples. FFPE, cfDNA, gDNA, fcDNA formats. Confidently verify your molecular oncology workflows.
We've updated our site with modern design and easier navigation, but you'll still find the same quality Dharmacon™ reagents and Mimix™ reference standards that you love.
Pan-Cancer 6-Fusion Panel FFPE RNA標準サンプルは、特性のよく明らかにされている臨床的意義のある細胞株由来の精度管理試料であり、融合遺伝子の検出を目的としたターゲットNGS RNA-Seq、endpoint reverse-transcription PCR(エンドポイントRT-PCR)、RT-qPCR およびRT-ddPCRアッセイの性能評価のために使用します。
各15 µm切片にはホルマリン固定パラフィン包埋(FFPE)された複数の細胞株のミックスが含まれており、トランスクリプトーム解析で6つのキナーゼ関連融合RNAが含まれていることが確認されています。
Product Highlights
フォーマット:FFPE
対象遺伝子:TPM3-NTRK1, QKI-NTRK2, ETV6-NTRK3, EML4-ALK, CCDC6-RET, SLC34A2-ROS1
固定方法:10%ホルマリン
切片サイズ:15 µm
ユニットサイズ:1 x 15 µm FFPE切片
保管温度:4℃
有効期限:product shelf life informationを参照してください。
検証済み変異:
| 5' Partner | 5' Partner Chromosome | 3' Partner | 3' Partner Chromosome | RNA Expression Level |
|---|---|---|---|---|
|
TPM3(Exon 7) |
1 |
NTRK1(Exon 10) |
1 |
≥100 Copies/ng |
|
QKI(Exon 6) |
6 |
NTRK2(Exon 14) |
9 |
≥100 Copies/ng |
|
ETV6(Exon 5) |
12 |
NTRK3(Exon 15) |
15 |
≥4 Copies/ng |
|
EML4(Exon 13) |
2 |
ALK(Exon 20) |
2 |
≥40 Copies/ng |
|
CCDC6(Exon 1) |
10 |
RET(Exon 12) |
10 |
≥4 Copies/ng |
| SLC34A2(Exon 4) |
4 |
ROS1(Exon 32) |
6 |
≥40 Copies/ng |
使用目的: アッセイの検証、およびアッセイの日常の品質・精度管理用(研究用試薬)
抽出可能なRNAまたは期待される収量:Maxwell RSC® RNA FFPE Purification Kit(Promega)を使用時、1切片あたり100 ng以上のRNA
RNA定量法:Qubit® RNA HSアッセイ(Thermo Fisher Scientific)
融合遺伝子発現:TPM3-NTRK1、QKI-NTRK2、ETV6-NTRK3、EML4-ALK、CCDC6-RET、およびSLC34A2-ROS1 RNA転写産物の発現は、QX200 ddPCRシステム(Bio-Rad)を使用した融合遺伝子特異的RT-ddPCRにより確認
RNAの品質:TapeStation High Sensitivity RNA ScreenTape Assay(Agilent)を使用時、期待されるDV200値は65%以上
We accept orders using the following methods.
Our online catalog and ordering system can be used to search our full menu of available Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.
If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order. We recommend that you use our online system to identify the products you require and their respective “HD” product codes.
To order offline we will require the following information:
If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please contact us.
Defined cell-line derived FFPE control material, verified as negative for TPM3-NTRK1, QKI-NTRK2, ETV6-NTRK3, EML4-ALK, CCDC6-RET and SLC34A2-ROS1 fusions