siGLO Cyclophilin B Control siRNA
Correlate fluorescence uptake and silencing activity with a single control
siGLO Cyclophilin B Control siRNA is a validated positive control, guaranteed to silence cyclophilin B in human, mouse, or rat cells. This control siRNA is chemically modified to significantly extend siRNA stability and fluorescence compared to conventional siRNAs similarly labeled with fluorophores.
Also known as peptidylprolyl isomerase B (PPIB), cyclophilin B is associated with the secretory pathway. This gene is abundantly expressed in most cells, and because it is non-essential, knockdown of the corresponding mRNA does not affect cell viability.
- Targets accession numbers NM_000942 (Human), NM_011149 (Mouse), or NM_022536 (Rat)
- Labeled with Cy3
- Absorbance/Emission Max is 547/563 nm
- A Cy3, Rhodamine or PE filter can be used
- Significantly extended intensity of the fluorescence signal (7 days or longer)
- Positive correlation of fluorescence with transfection efficiency and target gene silencing
To request a larger quantity or in vivo processing of this control product, please email Technical Support.
Cytoplasmic fluorescence confirms transfection with siGLO Cyclophilin B control siRNA
siGLO Cyclophilin B control (50nM) was transfected into HeLa cells with DharmaFECT 1 (0.15 µg/100µL well). After 48 hours, cells were washed with 1x PBS and stained with Hoechst 3342 nuclear dye (blue).
Functional knockdown using siGLO Cyclophilin B control siRNA
Cyclophilin B mRNA levels were quantified by RT-qPCR in HeLa cells 48 hours after transfection with DharmaFECT 1 (0.15 µg/100µL well) and 50nM of siGLO Cyclophilin B control, siGLO RISC-Free control, or siGLO Red Transfection Indicator.
The most broadly applicable DharmaFECT formulation for optimal siRNA or microRNA transfection into a wide range of cell types for successful RNAi experiments
Concentrated buffer solution recommended for resuspension and long-term storage of any short, double-strand, or single-strand synthetic RNA molecule. Dilute with RNase-free water prior to use.
Molecular grade water for dilution of 5x siRNA Buffer or resuspension of RNA. RNase-free to prevent degradation of RNA reagents and oligonucleotides.