Pro(tein) Validated Engineered Cell Lines  

Our Pro(tein) Validated Engineered Cell Lines are expertly verified with targeted antibodies to give you the confidence you need that our knock-out cell lines have both a loss of gene and protein expression, giving power to your functional studies.

Different Forms of Validation

Revvity has an extensive collection of HTRF™ (Homogeneous Time Resolved Technology) assays, a FRET-based, no-wash technology with a wide range of applications. HTRF assays provide key data for knockout cell line validation. A loss of fluorescence signal when comparing wild-type cells with engineered cells provides high levels of confidence that our knockout cell lines lack functional protein of the targeted gene.

BioLegend™ antibodies are designed to target unique epitopes on specific proteins, and they can be optimized for a variety of readouts, including western blot, flow cytometry, immunofluorescence, immunocytochemistry, and immunohistochemistry. Using these antibodies across different applications can validate our cell lines. A loss of signal when comparing wild-type to knockout cells demonstrates a loss of protein expression of target gene. 

Illustration of HTRF assay. Two antibodies, one with a donor and one with an acceptor fluorophore, bind to distinct epitopes on target proteins.

Explore our protein-validated collection

New cell lines added regularly.

Learn more about HTRF assays

Learn more about BioLegend antibodies