Single-strand RNA synthesis customization beyond the industry standards
Revvity's Dharmacon™ custom RNA synthesis goes beyond industry standards, offering unparalleled flexibility and capabilities. Researchers can synthesize long RNA oligos, highly modified RNA molecules, and unique RNA structures. A wide range of standard modification like phosphothioate linkages, psudouridine and fluorescent labels are available directly on the web, along with theraputically relevant modification like GalNAc clusters and 2'-O-methyl bases.
Our RNA synthesis is powered by our proprietary 2'-ACE chemistry that allows delivery of RNA in a protected form for enhanced stability and versatility in chemical modification. Purification options like HPLC and PAGE ensure high quality for demanding applications. Seamless online ordering with instant pricing and delivery estimates makes custom RNA synthesis easy, and for specialized needs beyond the online catalog, our scientific support team is ready to develop a custom solution.
Individual custom RNA
Multiple custom RNAs
Other custom RNA needs
Custom siRNA
Custom CRISPR guide RNA
Our RNA synthesis platform is built for researchers who need more - more flexibility, more precision, and more options than standard offerings provide.
Up to 140 bases long or more are supported to enable emerging technologies.
Choose from phosphorothioate linkages, 2'-O-methyl, 2'-fluoro, conjugation handles and fluorescent labels, and more options to enhance stability and function. A full list of modifications available on our web ordering tools can be found at our Bases and Modifications page.
Order individual RNAs or place bulk orders with consistent characteristics (scale, purification, and modifications).
Larger synthesis scales for in vivo and preclinical development.
Including modified bases not commercially available
Backed by 30+ years of oligo synthesis experience and trusted by researchers worldwide.
All oligos made by the Dharmacon Reagents team are examined by mass spectrometry. Additionally, ultra-HPLC analytical data and Mass-spec traces are available on request as an additional service.
Request modifications or customized purification and processing beyond the standard catalog or get design guidance from our team. Contact our Scientfic Support team today.
Standard modifications offered include:
Modified bases such as 2'-O-methyl bases
Pseudouridine (~U)
Fluorescent labels and quenchers
Terminal linkers and functional groups for conjugation chemistries
Phosphorothioate backbone modifications (*)
Oligo labeling options:
View our web-tool enabled Bases and Modifications
NOTE: Many other modifications are available upon request. The online ordering tools only include a subset of common modifications. For assistance please contact Scientific Support.
The ability to synthesize longer oligos is highly sequence-dependent due to the potential for secondary structure formation and the inherent difficulty for chemical synthesis of certain sequences.
Reduced overall yield is expected as oligo length increases.
| How today's scientists use RNA oligos | |
|---|---|
| Single-strand RNA may be used for | Applications for RNA may involve: |
| Custom microRNA inhibitors | in vitro or in vivo applications |
| Aptamers | Virology |
| Ribozymes | miRNA functional studies |
| tRNAs | X-ray crystallography, NMR |
| FISH/ISH probes | RNAi |
| FRET probes | Transcription / translation |
| RNA/DNA hybrids | Assay development |
| Custom RNA duplexes | Microarrays |
| Dicer substrates | RNA-mediated cellular mechanisms |
| Antisense oligos | RNA/protein interactions |
| Enzyme substrates | RNA chemistry |
Benefits of 2'-protected RNA:
Read more on our application page.
Protected RNA must be deprotected before use. You choose how:
| Option | Details | Cost |
|---|---|---|
| Deprotect in your lab | Simple 30-minute procedure; deprotection buffer included with order | No charge (uncheck 2’-Deprotect / Desalt box when checking out) |
| Deprotecting prior to shipping (default) | Receive your oligo ready-to-use after resuspension; select "2'-Deprotect / Desalt" at checkout | Small processing fee |
We routinely achieve 80-85% purity for unmodified single-strand RNA <35 nt. However, purification may be recommended when chemically synthesized RNA oligonucleotides are:
Please contact Scientific Support for information regarding recommendations for purification or purity estimates for unpurified material.
| Unprocessed | Desalt/Deprotect | PAGE | HPLC | In vivo | In vivo HPLC | |
|---|---|---|---|---|---|---|
| Deprotected | * | * | ||||
| Desalted | * | * | ||||
| Endotoxin tested | ||||||
| Sodium counter-ion exchange | ||||||
| Recommended for 3' or dually labeled RNA | ||||||
| Recommended for in vivo use |
* PAGE and HPLC purification options can be requested with or without Desalt / Deprotect
Watch our short video on how-to-use the custom single strand RNA synthesis tool
Try using our Dharmacon Oligo Sequence Converter.
Access our Ordering and calculation tools page for convenient webtools to support everything from designing your own siRNA or CRISPR guide against any organism to analyzing your PCR primers.
Revvity's Dharmacon Reagents team adheres to the guidelines in the United States Framework For Nucleic Acid Synthesis. For more information please see our self-attestation.
