- Advantages of Horizon's CRISPR-Cas9 Tools for gene editing
See the benefits of using Horizon’s CRISPR-Cas9 gene editing reagents. - Basic siRNA resuspension
Small interfering RNA (siRNA), crRNA and tracrRNA, synthetic microRNA mimics and inhibitors and custom RNA comes dried and must be resuspended before use. - Choosing a Dharmacon siRNA product line and format
Sometimes having too many choices is more difficult than having too few. We are here to help. - CRISPR-Cas9 experiment design
Choosing the right products for a CRISPR experiment - CRISPR-Cas9 screening
Advance in CRISPR-Cas9 screening - CRISPR Design Tool - library of custom guide RNAs
This video shows how to use the CRISPR Design Tool to design custom guide RNAs (available in synthetic crRNA, synthetic sgRNA and lentiviral formats) for your gene editing experiment. - CRISPR Design Tool for HDR
This quick tutorial will walk you through how to use the CRISPR Design Tool on the Dharmacon website to specifically design and order custom guide RNAs for your precise HDR-mediated experiment. - DharmaFECT Duo for simultaneous delivery of RNA and DNA
This video describes the use of DharmaFECT Duo transfection reagent to deliver both RNA and DNA in the same transfection - Edit-R CRISPR-Cas9 gene engineering platform
This video gives an overview of CRISPR-Cas9 gene editing, followed by the details of how the Edit-R™ CRISPR-Cas9 Gene Engineering Platform simplifies the gene editing workflow by using synthetic RNA
- HDR DNA Donor Designer: plasmid design
Learn how to design and build a custom plasmid for HDR insertion in your CRISPR experiment - HDR Donor Designer: tutorial for DNA insertion
The HDR Donor Designer tool can be used to efficiently design and order custom ssDNA donor oligos for insertion, removal, or replacement. - HDR Donor Designer: tutorial for DNA removal
The HDR Donor Designer can be used to efficiently design and order custom ssDNA donor oligos for insertion, removal, or replacement of genomic DNA with the CRISPR-Cas9 system. - HDR Donor Designer: tutorial for DNA replacement
The HDR Donor Designer can be used to efficiently design and order custom ssDNA donor oligos for insertion, removal, or replacement of genomic DNA with the CRISPR-Cas9 system. - How to measure viral titer using fluorescent colony counting
Viral titers are determined in HEK 293 cells, which are easy to transduce. Most other cells types are transduced with lower efficiencies than HEK 293. - How to use the Cherry-pick RNAi library plater
This short video demonstrates how to use the Cherry-pick RNAi library plater for creating a custom library of RNAi reagents. - Quick tip - How to import gene symbols into excel
This video provides a solution to a common problem encountered when pasting genetic data into Microsoft Excel - Quick tip - How to calculate viral titer
Calculating a functional viral titer may seem like a difficult task. - Quick tip - Speed up spin column purification
Very often when performing spin column purification, a small amount of wash buffer remains on the lip of the column after spinning it through the membrane. - Top considerations for RNAi and CRISPR-Cas9
Deciding which technology to use for loss-of-function studies? Learn about the key differences here!