Dharmacon™ reagents video series
- A Closer Look at Dharmacon 2.0 siRNAs Learn how we design our off-the-shelf siRNAs, why we periodically review them, and the benefits to using version 2.0 siRNAs
- What is inducible vs constitutive gene expression? Gene expression using plasmid vectors is either inducible or constitutive. Explore what this means using products from our CRISPRa portfolio as examples.
- A 2 minute intro to gene function studies Learn more about the trusted, high quality, Dharmacon solutions for gene function studies including RNAi, CRISPR, and gene expression technologies.
- CRISPR modulation applications Use CRISPR-level specificity to upregulate or repress your gene of interest using the Dharmacon CRISPR modulation platforms including CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) for functional genomic studies and discovery.
- Advantages of CRISPRi in 30 seconds Learn how CRISPR interference is an advantage for gene target identification and other applications.
- Dharmacon ON-TARGETplus siRNA reagents Build confidence in your loss-of-function studies with our ON-TARGETplus™ siRNA designed and modified for greater specificity.
- Learn about the DyadGuide dual-sgRNA Lentiviral Platform: how it works, it's uses, and who needs it.
Dharmacon™ Edit-R™ gene editing
- CRISPR gene editing See the benefits of using Dharmacon Edit-R CRISPR gene editing reagents and expert cell line engineering and screening services.
- CRISPR gene knockout experiment design Choose the right products for your specific CRISPR workflow, cell type and experimental goals.
- CRISPR vs RNAi Learn the benefits of each platform for targeted gene loss-of-function experiments.
- How do HAP1 KO cell lines work? Learn about the benefits of using human HAP1 knockout cells for disease modeling and antibody validation.
- What happens to the protein in KO cell lines? Learn about possible protein fates in HAP1 knockout cells.
- CRISPR video series
CRISPR background Learn about the critical steps for setting up a successful gene editing experiment.
Cell line optimization - Learn about methods for optimizing transfection efficiency.
Genetic variability considerations - Learn about considerations for genetic variability in your cell line.
Functional gene knockout - Learn about guide RNA design for highly specific and efficient functional gene knockout.
HDR mediated knock-in - Learn about guide RNA and donor design for HDR mediated knock-in.
Clonal screening - Learn screening strategies for clonal isolation.
Validation techniques - Learn about strategies for validating your edited cell line.
Dharmacon™ CRISPRmod™
- What is CRISPR interference or CRISPRi? Learn about Horizon's novel system for targeted gene down regulation at the transcriptional level.
Dharmacon™ custom oligo synthesis
- Custom Oligo Synthesis Tips Watch our 6-part video series for confidence designing and ordering complex oligos.
- Custom oligo synthesis capabilities Watch this 1 min video to see how our custom oligo synthesis services can can help accelerate your research
- How to use the Dharmacon custom siRNA synthesis tool Quickly generate a quote online for siRNA customized to your application.
- How to use the Dharmacon custom single strand RNA synthesis tool Quickly generate a quote online for custom RNA oligos.
- How to use the Dharmacon™ microRNA custom oligonucleotide ordering tool Watch this video for a tutorial on how to order custom synthetic miRNA.
Reference standards
- How to choose your reference standards See our portfolio of reference standards and where to use them in your diagnostic workflow.
Cell line engineering
- What happens to the protein in a knockout cell line? Learn the possible fates of a protein after CRISPR knockout
- Cell line engineering with Horizon See why you can trust our cell line engineering platform to deliver your next edited cell line project.
Tutorials
- How to request a quote See how to request a quote on the Horizon website in less than 60 seconds.
- Basic siRNA resuspension All synthetic siRNA, CRISPR guide RNA, microRNA mimics and inhibitors, and custom RNA reagents arrive dried and must be resuspended before use.
- Calculating functional titer Calculating a functional titer may seem like a difficult task. Here we show you step by step how to calculate functional titer.
- CRISPR Design Tool - order custom CRISPR guide RNA See how to use the CRISPR Design Tool to design custom guide RNAs (available in synthetic and lentiviral formats) for your gene knockout or HDR experiments.
- HDR Donor Design for DNA insertion: ssDNA donor oligo Learn how to design and order a custom ssDNA oligo for HDR insertion.
- HDR DNA Donor Designer: DNA plasmid design Learn how to design and order a custom DNA plasmid for HDR insertion.
- Building a custom screening library with the Cherry Pick Library Tool Learn how to turn your gene list into a custom mini-library for CRISPR or RNAi screening.
- Using the Pooled Tracking Worksheet to plan your CRISPR knockout screen This worksheet may be used with any of Horizon's pooled CRISPR screening libraries.
- What is Mass Spectrometry (MS)? A short video to help you understand what Mass Spectrometry (MS) is, why we use it and why/how customers can request MS for custom oligo synthesis.
- What is MOI and how does it affect lentiviral transduction experiments? See why multiplicity of infection is the key to successful transduction.
- What is fold representation and how does it affect pooled lentiviral screening? See why fold representation is key to successful pooled multi-gene screening with lentiviral CRISPR or shRNA libraries.