New and updated Dharmacon reagents and cell lines
Whether you're conducting single-gene studies or genome-wide screens, Revvity provides the most comprehensive portfolio of functional genomic tools available. Our Dharmacon™ solutions span the entire research spectrum—DNA, RNA, protein, and even cells lines—giving you precise control over gene modulation and editing to accelerate your path from discovery to therapeutic breakthrough.
Ready to advance your research? Explore our latest innovations designed to tackle today's most complex genomic challenges.
RNA interference
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Transfection-free gene silencing aligned with ever evolving transcriptome annotations. Accell™ 2.0 siRNA combines updated design algorithms with unique self-delivering chemical modifications to support gene silencing in primary cells and other difficult-to-transfect models. |
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Achieve potent on-target mRNA knockdown with siRNA designed against the modern genome. ON-TARGETplus 2.0 combines the trusted chemical modification pattern and proven performance you rely on, now with updated design and alignment to the latest RefSeq transcript annotations. |
CRISPR technologies
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Deliver two sgRNAs from a single lentiviral vector using a novel bidirectional U6 promoter. Available with all Dharmacon™ lentiviral CRISPR formats and technologies. |
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Strict-R is a novel dual-regulated inducible CRISPR system where transcription of the transgene is repressed and the transgene protein is unstable in the absence of small molecule inducers. This dual control dramatically reduces background activity while maintaining full activity, ensuring stringent control of ON and OFF state. |
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Purified ready-to-use Cas9 protein with a new and improved NLS composition enabling more efficient nuclear delivery for rapid RNP workflows.
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Comprised of our CRISPRmod synthetic sgRNAs pre-designed guides for synthetic CRISPRa applications available; these collections include curated gene family collections, fully customizable arrayed libraries, up to collections spanning the whole human genome; the first and only genome-scale collections of pre-designed CRISPRa sgRNAs available. |
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All Edit-R human sgRNA designs have been updated to the latest RefSeq in 2025 providing the most specific and genomically relevant guides for protein knockout. This update allows the Edit-R algorithm to target the latest genome annotations providing you with the best solution for your research. |
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NGS library prep kits offering complete screening-to-NGS solutions for all CRISPR lentiviral sgRNA pooled libraries.Learn more Lentiviral sgRNA pooled librariesAll-in-one lentiviral sgRNA whole genome pooled libraryCRISPRa All-in-one Lentiviral sgRNA Whole Genome Pooled LibraryCRISPRi All-in-one Lentiviral sgRNA Whole Genome Pooled Library |
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CRISPRmod synthetic sgRNAs for CRISPRa offer the first whole-genome scale set of pre-designed guides for synthetic CRISPRa applications available. These conveneient designs also match our lentiviral sgRNA designs enabling uniform and flexible gene-activiation studies like never before. |
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Cutting controls target safe harbor (AAVS1 and Rosa26) regions for genomic cuts without phenotypic changes. Validated for mismatch detection assays, they're perfect for confirming Cas9 activity in screening applications without functional consequences.
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New validated synthetic controls targeting the constant regions of the T-cell receptor for TRAC, TRBC, TRDC and available for both sgRNA and crRNA formats.
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Base editing technology
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Our Pin-point™ base editing reagents now include AI-engineered adenine deaminases from Profluent. Now both ABE and CBE reagents are available as catalog products for your proof-of-concept studies. |
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Our Pin-point™ base editing design tool makes it simple to create base editing sgRNAs that deliver reliable, high-efficiency gene knockouts.
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Transfection and transduction reagents
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LentiBOOST transduction enhancer can increase successful viral transduction in challenging to transduce cells or complex cellular engineering work, while preserving cell viability and minimizing the amount of viral particles required for your experiment. It's been tested with Dharmacon lentiviral reagents and is now available on our website. |
Engineered cell lines
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Our newest pre-engineered and ready-to-ship HAP1 cell lines for a selection of popular genes, including SMAD5, KLF6, ATM, SYK, NPM1, BRCA1, PARP1, RPTOR, BCL2L1, SKP2, PUDP, KCTD7, DNAJC5, CTSF, and KLHL22.
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