RNAi Controls
siRNA, shRNA, miRNA and lncRNA experimental controls for gene knockdown and microRNA study
Achieving reliable, reproducible gene silencing or microRNA modulation requires more than an optimized RNAi reagent, it demands a well-designed control strategy. Our RNAi controls are validated to support every stage of experimental design, from confirming delivery efficiency to distinguishing sequence-specific silencing from non-specific effects. Whether optimizing transfection conditions or characterizing knockdown across cell types, these controls provide the confidence needed to accurately interpret results and draw meaningful conclusions.
siRNA Controls
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ON-TARGETplus controls
Validated positive and non-targeting negative controls with proprietary modifications for superior specificity. -
ON-TARGETplus 2.0 controls
Validated positive and non-targeting negative controls with proprietary modifications for superior specificity. -
Accell 2.0 controls
Optimize Accell 2.0 siRNA delivery using validated fluorescent or unlabeled positive and negative controls. Kits available. -
siGENOME controls
Validated positive, negative, and RISC-Free® controls to assess all aspects of your RNAi experiment. Kits available. -
siGLO controls
Fluorescent controls for visualizing transfection success - choose from positive siRNAs or transfection indicators. -
Reporter genes, siSTABLE and other controls
siSTABLE-modified controls, reporter gene siRNAs (GFP, Luciferase), and more for specialized experimental needs. -
Lincode controls
Endogenous lncRNA positive and non-targeting control reagents for high-confidence experiments.
shRNA controls
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SMARTvector lentiviral shRNA controls
Positive and negative controls with choice of matching promoters and two reporters. -
SMARTvector inducible lentiviral controls
Inducible positive and negative controls with choice of matching promoters and two reporters. -
GIPZ lentiviral shRNA controls
Positive and negative controls for well-designed RNAi experiments. -
TRIPZ inducible lentiviral shRNA controls
Inducible positive and negative controls for well-designed RNAi experiments. -
TRC lentiviral shRNA controls
Positive and negative controls for well-designed RNAi experiments.
microRNA controls
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miRIDIAN microRNA mimic controls
Fluorescently labeled transfection control and complete portfolio of positive and negative controls to optimize microRNA mimic experiments. -
miRIDIAN microRNA hairpin inhibitor controls
Fluorescently labeled transfection control and complete portfolio of positive and negative controls to optimize microRNA inhibitor experiments.
Types of controls
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Positive controls
Validated reagents to monitor and optimize delivery efficiency across replicates, conditions, or cell types - matched to every pre-designed RNAi product available.
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Non-targeting controls
Establishes a baseline to confirm target-specific knockdown and rule out non-specific effects. Available matched to all of our pre-designed RNAi products.
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RISC-Free™, Empty Vector or mock transfection
Detects non-RNAi cellular effects from transfection or transduction, such as toxicity or off-target gene expression changes.
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Fluorescent controls
Dye-labeled indicators, including nuclear-localizing variants, to correlate signal intensity with optimal transfection conditions.
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Reporter gene controls
Control siRNAs for commonly used reporter systems (GFP, Luciferase, etc.) that have been experimentally validated for functionality.
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TOX transfection control
TOX Transfection Control provides a simple method for assessing transfection efficiency and is cytotoxic only when productively delivered into cells.
Which RNAi control is best for you?
Use the guide below to help determine which RNAi control is best for your research needs.
| Product Line | Endogenous Positive Control | Exogenous Positive Control | Negative Control | Fluorescent Label | Control Kits | |
|---|---|---|---|---|---|---|
| siRNA | siGenome siRNA | Lamin A/C, PPIB, GAPD | Non-targeting, RISC-Free | |||
| ON-TARGETplus 2.0 siRNA | PPIB, GAPD | Non-targeting | ||||
| ON-TARGETplus siRNA | PPIB, GAPD | Non-targeting | ||||
| Accell 2.0 siRNA | PPIB, GAPD | eGFP | Non-targeting | |||
| siSTABLE siRNA | PPIB, GAPD | Non-targeting | ||||
| siGLO siRNA | Lamin A/C, PPIB | RISC-Free | ||||
| siGLO Transfection Indicators | ||||||
| Reporter gene controls | RLuc, FLuc, GPF, beta-gal, GL2 and GL3 Luciferase | |||||
| Lincode siRNA | lincode | GAS5 | Non-targeting | |||
| shRNA | SMARTvector Lentiviral shRNA | PPIB, GAPD | Luciferase | Non-targeting, empty vector | ||
| SMARTvector Inducible shRNA | PPIB, GAPD | Non-targeting | ||||
| GIPZ Lentiviral shRNA | EG5, GAPD | Non-targeting, empty vector | ||||
| TRIPZ Inducible Lentiviral shRNA | GAPD | Non-targeting, empty vector | ||||
| TRC Lentiviral shRNA | eGFP | Empty vector | ||||
| microRNA | shMIMIC Lentiviral microRNA | PPIB, GAPD | Non-targeting, empty vector | |||
| shMIMIC Inducible microRNA | PPIB, GAPD | Non-targeting | ||||
| miRIDIAN microRNA Mimics | miR-122, 3' UTR controls | Negative Control | ||||
| miRIDIAN microRNA Hairpin Inhibitors | Aldolase A, PPIB and GAPD | Negative Control |
Control kits
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Accell 2.0 Control siRNA Kits
A comprehensive set of controls designed to assess and optimize Accell 2.0 siRNA performance in your cell type, supporting confident interpretation of gene silencing results. These controls are matched with Accell 2.0 siRNA designs, helping maintain consistency with your experimental reagents.
Each kit contains:
- Accell 2.0 GAPD Control siRNA (Human, Mouse)
- Accell 2.0 Cyclophilin B Control siRNA (Human, Mouse)
- Accell 2.0 non-targeting siRNA #1
- Choice of Green or Red Accell 2.0 Non-targeting siRNA (for uptake visualization)
- 5X siRNA Buffer (optimized for Accell 2.0 delivery)
- Accell siRNA Delivery Media
Designed to support optimization and experimental control, these kits provide a strong foundation for transfection-free gene silencing workflows in difficult-to-transfect cells
Species Accell 2.0 Control siRNA Kits (Green) Human, Mouse Accell 2.0 Control siRNA Kits (Red) Human, Mouse -
siGENOME control siRNA kits
A comprehensive array of siRNA control reagents for use in any siRNA experiment.
Each siGENOME Controls Basic Kit contains 5nmol each of:
- siGENOME Lamin A/C Control siRNA (Human, Mouse, or Rat)
- siGENOME Cyclophilin B Control siRNA (Human, Mouse, or Rat)
- siGENOME Non-targeting siRNA #2
- siGENOME Non-targeting siRNA #5
- siGENOME RISC-Free Control siRNA
- siGLO Green Transfection Indicator
- siGENOME Non-targeting siRNA Pool #2
Each siGENOME Controls Complete Kit additionally contains:
- Three additional siGLO control siRNAs
- TOX Transfection Control
Species siGENOME Controls Basic Kit Human, Mouse, Rat siGENOME Controls Complete Kit Human, Mouse, Rat
