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Custom miRNA synthesis

Customized microRNA reagents for any research application

Easily order your ideal Dharmacon™ miRIDIAN™ microRNA mimic or inhibitor - from sequence to synthesis - with flexibility, scale, and chemical expertise. Whether modifying an existing microRNA mimic or inhibitor, targeting a novel microRNA, or requesting an Accell™ microRNA inhibitor or stabilized mimic for special applications, our team can support your research.

Image representing microRNA synthesis

Watch our step-by-step video to learn how to use the custom miRNA ordering tool.

How we can help you study microRNAs.

Use our Dharmacon custom miRNA mimic ordering tool to:

  • Easily apply our proprietary miRIDIAN mimic modification pattern to your microRNA sequence.

  • Choose from multiple scales and processing options.

  • Access a wide variety of 5' and 3' chemical modifications.

  • Scale up for in vivo and large-scale studies.

For further customization of microRNA mimics and inhibitors our teams can help:

  • Order custom self-delivering Accell inhibitors or stabilized miRNA mimics for your assay.

  • Apply end modifications to mimics or inhibitors to assist in your assay needs.

  • Request large-scale production of microRNA reagents.

Supporting your microRNA needs

  • Extended options for enhancing your microRNA mimic or inhibitor
    Product Description Best Application
    Custom miRIDIAN Mimics Apply proprietary modification pattern to your sequence Novel targets, alternative species
    Stabilized Mimics Add stabilizing modifications for nuclease-rich environments in vivo, serum-containing conditions
    Accell™ Inhibitors MicroRNA inhibitor with Accell modifications for reagent-free delivery Hard-to-transfect cells, primary cells
    Custom Hairpin Inhibitors Tailored inhibitors for specialized knockdown Non-standard targets, mismatch studies

    Contact Scientific support for information and quoting assistance.

  • Advantages of custom synthesis for miRNA
    • Target any species - go beyond human, mouse, and rat to alternative model organisms
    • Introduce mismatches - study microRNA biomechanics and seed region function
    • Scale for animal studies - in vivo processing and large-scale synthesis available
    • Broad chemical flexibility - apply a wide range of 5' and 3' modifications to suit your experimental design

Helpful webtools

  • Convert your sequence from other common oligo shortcodes with our webtool

    Need help entering your sequence using our oligo shortcode? Try using our Dharmacon Oligo Sequence Converter.

  • Ordering and calculation tools

    Access our Ordering and calculation tools page for convenient webtools to support everything from designing your own siRNA or CRISPR guide against any organism to analyzing your PCR primers.

  • Revvity's adherence with the United States Framework For Nucleic Acid Synthesis Screening

    Revvity's Dharmacon Reagents team adheres to the guidelines in the United States Framework For Nucleic Acid Synthesis. For more information please see our self-attestation.

MicroRNA applications

Modulating endogenous miRNA targets with miRIDIAN microRNA mimics and inhibitors

Application note

MicroRNAs (miRNAs) have been shown to regulate gene expression through both translational attenuation and messenger RNA (mRNA) degradation.

Reciprocal microRNA modulation identifies gene targets in a biological context

Application note

Through their effect in down-regulation of gene transcripts, microRNAs play an important role in a range of biological processes such as development.

microRNA targets in stem cell differentiation

Application note

MicroRNAs are non-coding RNAs that provide critical translational regulation within the cell.

Screening microRNAs for determinants of osteogenesis

Application note

MicroRNAs (miRNAs) are endogenous non-coding RNAs that modulate gene expression through the RNA interference pathway.

4 great reasons for working with lentiviral microRNAs!

Featured article

Learn quickly how to maximize the success of your next microRNA experiment and accelerate your studies.

In vivo RNAi: Biodistribution, delivery, and applications.

Technical note

Learn about important considerations for in vivo studies with RNAi

Alternative miRNA design for therapeutic RNAi applications

Featured article

Design and performance implications of miRNA tools in vivo.

SMARTchoice Inducible shMIMIC Lentiviral microRNA enables you to do new things with expressed microRNAs

Featured article

The only INDUCIBLE multi-promoter, multi-marker, native microRNA context solution.

Featured resources

Protocol - Ethanol precipitation of RNA oligonucleotides

This protocol is for Ethanol Precipitation of RNA Oligonucleotides.

Protocol - Reverse phase cartridge desalting of RNA oligonucleotides

Protocol for desalting RNA oligos using reverse phase cartridges, including protocol for cartridge equilibration, washing, and eluting from cartridge.

Protocol - TCEP reaction for thiol-modified siRNA/RNA oligonucleotides

Protocol to deprotect (reduce) oxidized thiol-modified siRNA and RNA oligonucleotides.

Recommended reading

Publication list highlighting Dharmacon reagent development and collaborations across CRISPR-Cas9, RNAi, and oligo synthesis.

Protocol - Basic siRNA resuspension

This protocol outlines resuspension of siRNA and includes answers to frequently asked questions.

Protocol - DharmaFECT™ siRNA transfection

A protocol for use of DharmaFECT transfection reagents to deliver siRNA (or microRNA) into cultured mammalian cells.

SDS (US) RNA oligonucleotides

North American standard safety data sheet for RNA oligonucleotides.

SDS (EU) RNA oligonucleotides

European Union standard safety data sheet for RNA oligonucleotides.

How can we help you?

We are here to answer your questions.

Contact us